Tag Archives: anther culture

204-209 N. Burbulis, A. Blinstrubienė, R. Kuprienė and L. Žilėnaitė
Effect of genotype and medium composition on flax (Linum usitatissimum L.) anther culture
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Effect of genotype and medium composition on flax (Linum usitatissimum L.) anther culture

N. Burbulis, A. Blinstrubienė, R. Kuprienė and L. Žilėnaitė

Department of Crop Science and Animal Husbandry, Lithuanian University of Agriculture,Studentu St. 11, LT-53361 Akademija, Kaunas distr. Lithuania;e-mail: natalija.burbulis@lzuu.lt

Abstract:

Flax (Linum usitatissimum L.), a member of the family Linaceae, is an important crop in Europe for the production of both oil and fibre. Organic agriculture is based on minimizing the use of external inputs and avoiding the use of synthetic fertilizers and pesticides, therefore resistant genotypes are preferable in an organic agriculture system. Breeding flax using haploid techniques allows breeders to develop new varieties in a shorter time period. However the overall efficiency of plant regeneration is not efficient, therefore identification of responsible genotypes and improvements of protocols are prerequisites for applied breeding programs. The effect of the combination of genotype and growth regulators on callus induction and shoot regeneration in anther culture of flax was investigated. Anther culture response in nine flax cultivars was studied, and four responsible genotypes have been selected. The results suggested that specific combinations of growth regulators must be designed for each genotype. The highest rate of shoots per plant has been obtained in second subculture.

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