Tag Archives: BIO-PCR

151-154 D. Burokienė
Early detection of Clavibacter michiganensis subsp. Michiganensi in tomato seedlings
Abstract |

Early detection of Clavibacter michiganensis subsp. Michiganensi in tomato seedlings

D. Burokienė

Institute of Botany, Žaliųjų Ežerų g. 49, LT-08406 Vilnius, Lithuania;e-mail: daiva@botanika.lt

Abstract:

The investigations of Clavibacter michiganensis subsp. Michiganensi in tomato seedlings were carried out using the BIO-PCR method. One to two (1–2) colonies were already detectable with species-specific primers CMM5 and CMM6 using Clavibacter michiganensis subsp. michiganensis bacteria in plant seedlings. The method allowed detection of bacteria at a distance of 8 cm farthest from the inoculation site in 95% of samples 3 days after treatment, at the earliest. Plant seedlings approximately 40 cm high were infected at full length after 9 days. Experimental results indicated the possibility of detecting the pathogen in very early stages of infection. Therefore, the BIO-PCR method was a highly specific, rapid and reliable detection technique, which might help to control the spread of the pathogen to healthy tomato plants.

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