Tag Archives: Fragaria ananassa Duch.

335-339 R. Rugienius, T. Siksnianas, V. Stanys, D. Gelvonauskiene and V. Bendokas
Use of RAPD and SCAR markers for identification of strawberry genotypes carrying red stele (Phytophtora fragariae) resistance gene Rpf1
Abstract |

Use of RAPD and SCAR markers for identification of strawberry genotypes carrying red stele (Phytophtora fragariae) resistance gene Rpf1

R. Rugienius*, T. Siksnianas, V. Stanys, D. Gelvonauskiene and V. Bendokas

Lithuanian Institute of Horticulture, Babtai LT-54333, Kaunas distr. Lithuania
*Corresponding author; e-mail: r.rugienius@lsdi.lt

Abstract:

Phytopthora fragariae is responsible for strawberry red stele disease that spreads in almost all areas where strawberries are grown. RAPD markers that were developed previously for the strawberry Rpf1 gene are difficult to reproduce and preferentially, therefore, should be converted into sequence characterized amplified region (SCAR) markers. The aim of our study was to develop new SCAR markers, associated with Rpf1, to make a comparative study of RAPD and SCAR markers, and to select strawberry varieties and seedlings carrying the Rpf1 gene. A SCAR marker was constructed based upon RAPD marker OPO-16C, linked to the susceptibility allele of the Rpf1 gene. Following the cloning and sequencing of this marker we were able to design SCAR primers specific to this gene. Utilizing newly developed SCAR markers it was shown that resistant varieties like ‘Anapolis’, ‘Redgauntlet’ contain Rpf1 region. Common varieties ‘Elsanta’, ‘Kama’, ‘Venta’, ‘Senga Sengana’ did not exhibit this gene. The segregation rate of seedling populations (susceptible x resistant) by this marker was approximately 1: 1.

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