Bacterial endophytes of grapevine (Vitis vinifera L.) as promising tools in viticulture: isolation, characterization and detection in inoculated plants
¹All-Russia Research Institute for Agricultural Microbiology, Shosse Podbelskogo 3, Pushkin, RU 196608 St. Petersburg, Russia
²ITMO State University
³St. Petersburg State Technological Institute, Moskovsky prospect 26, RU 190013 St. Petersburg, Russia
*Correspondence: avsherbakov@bisolbi.ru
Abstract:
Bacterial endophytes may positively influence the host plants, and the search for new strains with beneficial properties is a promising research direction. We isolated culturable endophytic bacterial strains from cuttings of grapevine of four cultivars, identified and characterized their physiological properties and studied colonization process and localization sites of the introduced DsRed-labeled strain. The taxonomic diversity of microorganisms isolated from the inner tissues of grapevine was identified based on the analysis of the 16S rRNA gene fragments. Several promising strains of endophytic bacteria were isolated. DsRed+ phenotypes were obtained by transformation. Their introduction into grapevine plants made it possible to reveal their localization in the plant vascular tissues.
Key words:
16S rRNA gene, bacterial endophytes, colonization activity, CSLM, DsRed- labeling, grape (Vitis vinifera L.)