Tag Archives: in vitro

136-146 I. Lignicka, A. Balgalve and A.M. Zīdere-Laizāne
Zinc content in different muesli samples
Abstract |
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Zinc content in different muesli samples

I. Lignicka*, A. Balgalve and A.M. Zīdere-Laizāne

Felici LLC, Rigas gatve 8, LV-2164 Adazi, Adazu novads, Latvia
*Correspondence: ilva.lignicka@musli.lv

Abstract:

There is no specialized zinc storage system in the body, therefore there must be a daily intake of zinc to achieve a steady state. Long-term zinc deficiency due to inappropriate nutrition may result in immunological or autoimmune diseases. The aim of this study was to develop muesli with naturally high zinc content. Zinc is found in various plant-based foods as grain flakes and seeds which often are one of the raw materials for muesli. Muesli is one of the grain-based food trends nowadays as people`s life habits are changing due to fast-paced life, still, it is important to obtain all nutrients. According to zinc content in raw materials five different muesli samples were prepared, from all samples, muesli with the highest zinc content with 3.80 mg 100 mg-1 was chosen for further analysis. Zinc bioavailability is assessed through the determination of absorbability. The kinetic in vitro intestinal digestion suggests how much zinc is released during digestion and could be absorbed in the small intestine. The results show that during digestion approximately 22% of zinc was absorbed in the small intestine. Such characteristics of in vitro digestion test shows that by one meal (50 g of muesli) it is possible to replenish our body zinc level by 0.42 mg. To check additional nutrient content in selected muesli samples different mineral and vitamin analyses were done. Mineral and vitamin content in the muesli sample was calculated according to their content in raw materials. Per portion, muesli is source of iron, magnesium, phosphorus, zinc, vitamin B1, vitamin B2, vitamin B3, vitamin B6 and vitamin E.

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51-58 R. Sestras, E. Tamas, D. Pamfil, L. Mihalte,A. Sestras, L. Chis and C. Qin
The influence of the genotype upon the in vitro and in vivo growth of greenhouse carnations
Abstract |

The influence of the genotype upon the in vitro and in vivo growth of greenhouse carnations

R. Sestras¹, E. Tamas¹, D. Pamfil¹, L. Mihalte¹,A. Sestras², L. Chis² and C. Qin³

¹University of Agricultural Sciences and Veterinary Medicine, Faculty of Horticulture, 3-5Manastur St., 3400 Cluj-Napoca, Romania, e-mail: rsestras@usamvcluj.ro
²Horticultural Research Station, 3-5 Horticultorilor St., Cluj-Napoca, Romania
³School of Life Science, Shanghai University, Shanghai, 200436, China

Abstract:

Carnations are familiar, widespread, and are among the most popular cut flowers. There is great diversity of greenhouse cultivars belonging to Dianthus caryophyllus L, habitually multiplied through cuttings or micro-propagation. In order to establish whether or not there is a connection between the vigour of the plants technically mature from the greenhouse and their in vitro growth, several traits of greenhouse carnation, grown both in vivo and in vitro were analysed in five cultivars (Polka, Tanga, Dark Tempo, Delphi and Indios). The influence of the genotype upon the vigour of the plants and upon the characteristics of the greenhouse flowers, as well as upon some features of the in vitro growth of the plantlets, was significant. The variability under in vivo conditions ranged from 5.0 to 17.8%, while the characteristics analysed in vitro showed a large span of variability values (s% = 7.9–51.0). Overall, the heritability showed high values for the analysed characteristics, both under in vivo (H2 = 0.660–0.949) and in vitro (H2 = 0.502–0.946) conditions. No statistically ensured correlations were recorded between the plant growth under in vivo conditions and of those with the same genotype under in vitro conditions; therefore the greater vigour of some genotypes from the greenhouse did not imply their more accentuated growth in vitro. The cultivars conspicuous for their superior characteristics will be used as genitors within the improvement programmes and also recommended to be tested for inclusion in the Official Catalogue of Plants.

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