Tag Archives: plant growth regulators

1747-1755 D.A Egorova, O.I. Molkanova, Yu.N. Gorbunov, A.A. Gulevich and E.N. Baranova
Some features of cultivating different Chamaenerion angustifolium (L.) Scop. forms in vitro
Abstract |

Some features of cultivating different Chamaenerion angustifolium (L.) Scop. forms in vitro

D.A Egorova¹, O.I. Molkanova¹, Yu.N. Gorbunov¹, A.A. Gulevich² and E.N. Baranova¹²*

¹N.V. Tsitsin Main Botanical Garden of Russian Academy of Sciences, Botanicheskaya
street 4, RU127276 Moscow, Russia
²All–Russian Scientific Research Institute of Agricultural Biotechnology, Timiryazevskaya street 42, RU127550 Moscow, Russia
*Correspondence: greenpro2007@rambler.ru

Abstract:

Chamaenerion angustifolium (L.) Scop. characterized by a wide range of economically useful properties. White-flowered form of Ch. angustifolium (L.) Scop. Is extremely rare in nature. At the same time, it is promising as a source of biologically active substances and as a highly decorative plant. The optimal way to reproduce this form is clonal micropropagation. Methods for obtaining Ch. angustifolium in vitro were developed, as well as the optimal selection timing of starting material for micropropagation was determined. In addition, the effect of a mineral composition of nutrient medium and plant growth regulators on the regeneration of microshoots was studied. The highest values of morphometric parameters were achieved on MS medium (Murashige & Skoog, 1962) supplemented with 0.5 mg L-1 BAP. The multiplication factor of the lilac–flowered form was 8.4 ± 0.2, of the white-flowered form – 9.2 ± 0.6. Comparative analysis of morphometric parameters during cultivation of Ch. angustifolium showed no significant difference between the lilac-flowered and white-flowered forms. The effect of antioxidants on the growth and development of regenerants has been shown. The most optimal nutrient medium for clonal micropropagation of the lilac-flowered form was MS medium (Murashige & Skoog, 1962) containing 0.5 mg L-1 of BAP, 50 mg L-1 of ascorbic acid and 50 mg L-1 of citric acids and for micropropagation of the white–flowered form it was the medium, containing 100 mg L-1 PVP.

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103-112 Т.А. Kalinina, О.А. Vysokova, L.А. Khamidullina, А.А. Kochubei, О.Е. Cherepanova and Т.V. Glukhareva
The effect of ethyl 5′-(4-methoxybenzoyl)-5′,7′-dihydrospiro[cyclopentane-1,6′-[1,2,3]triazolo[5,1-b][1,3,4]thiadiazin]-3′-carboxylate on Pinus sylvestris L. seed germination
Abstract |
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The effect of ethyl 5′-(4-methoxybenzoyl)-5′,7′-dihydrospiro[cyclopentane-1,6′-[1,2,3]triazolo[5,1-b][1,3,4]thiadiazin]-3′-carboxylate on Pinus sylvestris L. seed germination

Т.А. Kalinina¹, О.А. Vysokova¹, L.А. Khamidullina¹², А.А. Kochubei³, О.Е. Cherepanova³ and Т.V. Glukhareva¹*

¹Ural Federal University named after the first President of Russia B.N. Yeltsin, Mira street 19, RU620002 Ekaterinburg, Russia
²Postovsky Institute of Organic Synthesis UB RAS, Sofia Kovalevskaya street 22, RU620990 Ekaterinburg, Russia
³Botanical Garden UB RAS, 8 Marta street 202a, RU620144 Ekaterinburg, Russia
*Correspondence: taniagluhareva@yandex.ru

Abstract:

Plant growth stimulators are capable of enhancing both agricultural output and the rate of plant maturation, consequently improving the total crop yield and increasing their resistance to disease and adverse environmental conditions. This is why such compounds are used in the cultivation of both agricultural and ornamental plants. The aim of this work was to study the effect of ethyl 5′-(4-methoxybenzoyl)-5′,7′-dihydrospiro[cyclopentane-1,6′-[1,2,3]triazolo[5,1-b][1,3,4]thiadiazin]-3′-carboxylate on Pinus sylvestris L. seed germination. The article describes the synthesis of ethyl 5′-(4-methoxybenzoyl)-5′,7′-dihydrospiro[cyclopentane-1,6′-[1,2,3]triazolo[5,1-b][1,3,4]thiadiazin]-3′-carboxylate and the data from spectral and X-ray crystal analysis. The results of the experimental stimulation of Pinus sylvestris L. seed germination using spiro-1,2,3-triazolo[5,1-b]1,3,4-thiadiazine are given compared to the commercially available phytohormones thidiazuron and 6-benzylaminopurine. The estimation of germination, vitality, healthy seed ratio and cotyledon length indicated that the tested compound at a concentration of 0.5 mg L-1 had an effect similar to 6-benzylaminopurine: indeed, the speed of germination and fungal invasion rate exceeded the effect of 6-benzylaminopurine.

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397-401 S. Supronienė, O. Auškalnienė, Z. Dabkevičius and A. Mankevičienė
The effects of growth regulators on spring barley (Hordeum vulgare L.) morphological indicators and grain contamination with fungi and mycotoxins
Abstract |
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The effects of growth regulators on spring barley (Hordeum vulgare L.) morphological indicators and grain contamination with fungi and mycotoxins

S. Supronienė, O. Auškalnienė, Z. Dabkevičius and A. Mankevičienė

Lithuanian Institute of Agriculture, Instituto str. 1, LT–58344 Akademija, Kedainiu distr.,Lithuania; e-mail: skaidre@lzi.lt, ona@lzi.lt,

Abstract:

The effects of growth regulators Modus 250 EC (trinexapac–ethyl 250 g l-1) and Cerone 480 (etefon 480 g l-1) on the morphological characteristics of the spring barley varieties Henni and Luokė and on grain contamination with fungi and mycotoxins were studied at the Lithuanian Institute of Agriculture in 2004 and 2005.Spring barley was sprayed with the retardant Modus at growth stages 32–33 BBCH andwith the retardant Cerone at – 39–43 BBCH. Plant morphological measurements were made before harvesting. Percent of internal grain contamination with fungi was determined. The concentrations of the mycotoxins deoxynivalenol (DON), T-2 toxin and zearalenone (ZEN) in grain were measured by ELISA method, using Neogen diagnostic mycotoxin determination tests.The retardants reduced plant height from 66.0 to 58.0 cm, length of the last node from19.4 to 17.9 cm for the variety Henni, and from 74.1 to 63.8 cm, and from 22.4 to 16.9 cm, respectively, for the variety Luokė compared with the unsprayed plants.The content of Fusarium-affected grain in the retardant-sprayed and not appliedtreatments was similar, however, Fusarium avenaceum (Fr.) Sacc was more prevalent in the barley sprayed with retardants. F. avenaceum, F. sporotrichioides Sherb., F. poae (Peck) Wollenw, F. tricinctum (Corda) Sacc., F. culmorum (W. G. Smith) Sacc. dominated in the grain samples in 2005.The contents of DON, ZEN and T-2 toxin determined were low, however, higher contentswere identified for barley sprayed with the retardants.

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