Tag Archives: Rhizomania

475-478 M. Žižytė, J. Staniulis and I. Zitikaitė
Identification of Beet necrotic yellow vein virus isolate detected in Lithuania
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Identification of Beet necrotic yellow vein virus isolate detected in Lithuania

M. Žižytė, J. Staniulis and I. Zitikaitė

Institute of Botany, Žaliųjų Ežerų 49, LT-08406 Vilnius, Lithuania;e-mail: marija.zizyte@botanika.lt

Abstract:

Sugar beet plants with rhizomania symptoms in their roots were detected in the central region of Lithuania. The study was undertaken to ascertain the possible presence of beet necrotic yellow vein virus (BNYVV) in the rootlets. DAS-ELISA, lateral flow tests, electron microscopy and mechanical transmission to indicator plantsconfirmed the presence of BNYVV. BNYVV was detected in leaf tissue of indicator plants with local lesions, and products of the expected size were amplified by RT-PCR, immunocapture RT-PCR (IC-RT–PCR) and nested PCR (nPCR) (500 bp for RT-PCR and 326 bp for nPCR). This is the second location where the presence of BNYVV quarantine pathogen in Lithuania has been proved.

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