Extraction of biologically active compounds from fruit, berry and grain Grist using ViscoStar 150L enzyme complex
¹International Research Centre ‘Biotechnologies of the Third Millennium’, ITMO University, St. Lomonosova 9, RU 191002 Saint-Petersburg, Russian Federation
²Federal State-Funded Scientific Establishment ‘All-Russia Research Institute for Food Additives’ (FGBNU VNIIPD), Chair of Food Microingredients’ Processing, 55 Liteinii ave., RU 191014 Saint-Petersburg, Russian Federation
This work aimed to evaluate the efficiency of treating plant tissue with enzymatic agent ViscoStar 150L for the extraction of biologically active compounds. In the current study the screening of extraction methods from citrus fruit peels (grapefruit, lemon, orange) was performed. The samples treated with enzymatic agent ViscoStar 150L showed better extraction results than the traditional ethanol/water extraction method. Citrus peels’ extracts assayed for antioxidant activity (determined as ferric reducing antioxidant power – FRAP) decreased in the following order: grapefruit > orange > lemon. The enzymatic agent ViscoStar 150L proved to have a positive synergic effect on juice yield from cowberry previously treated with a complex pectolytic enzymatic agent. The enzymatic agent ViscoStar 150L proved to have a synergic effect on grain grist mashes previously treated with an amylolytic enzymatic agent, the inhibitor activity of the compounds produced by actinomycetic microorganisms grown on substrates based on these mashes being higher than that of previously known inhibitors.