Tag Archives: colostrum

801-806 A. Sats, H. Mootse, S. Pajumägi, A. Pisponen, V. Tatar and V. Poikalainen
Estimation of Particle Size Distribution in Bovine Colostrum Whey by Dynamic Light Scattering (DLS) Method
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Estimation of Particle Size Distribution in Bovine Colostrum Whey by Dynamic Light Scattering (DLS) Method

A. Sats*, H. Mootse, S. Pajumägi, A. Pisponen, V. Tatar and V. Poikalainen

Estonian University of Life Sciences, Institute of Veterinary Medicine and Animal Sciences, Department of Food Science and Technology, Kreutzwaldi ⁵⁶/⁵, EE⁵¹0¹⁴ Tartu, Estonia; *Correspondence: andres.sats@emu.ee

Abstract:

Colostrum whey consist bioactive compounds in considerable concentration. For isolation of these compounds the particle size has crucial importance. The aim of this work was to study possibilities of using dynamic light scattering method – DLS (Malvern Zetasizer Nano ZS) for colostrum whey particle size distribution estimation. The first and second milking colostrum samples were skimmed by centrifugal separation and casein of these was enzymatically coagulated by rennet (chymosin). Obtained whey was diluted (¹:²00) by distilled water and filtered (cut-off 0.⁴⁵ m) to get probes for estimation of particle size. Particle size distribution in colostrum whey probes had maximally three peaks and polydispersity indices from 0.¹⁵⁷ to 0.⁵⁴¹. Prevailing peak of the distribution was found at size from ¹⁴⁴ to ²¹0 nm, which apparently corresponds to hydrodynamic diameter of immunoglobulin IgG¹.

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415-420 M-L. Kütt, M. Malbe and J. Stagsted
Nanostructure-Assisted Laser Desorption/Ionization (NALDI) for Analysis of Peptides in Milk and Colostrum
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Nanostructure-Assisted Laser Desorption/Ionization (NALDI) for Analysis of Peptides in Milk and Colostrum

M-L. Kütt¹⋅², M. Malbe¹ and J. Stagsted³

¹Department of Agricultural Products, Estonian Research Institute of Agriculture,Teaduse 13, EE75501 Saku, Estonia; e-mail: maryliis.kytt@eria.ee; marge.malbe@eria.ee
²Department of Food Processing, Faculty of Chemical and Materials Technology,Tallinn University of Technology, Estonia
³ Department of Food Science, Aarhus University, Blichers Allé 20, Postboks 50D K-8830 Tjele, Denmark; e-mail: Jan.Stagsted@agrsci.dk

Abstract:

Several bioactive proteins have been identified in colostrum and milk. However there are needs for development of technologies to identify and purify low molecular weight (LMW) peptides with bioactivity. The most used method is Matrix Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS), but matrix suppression often prevents detection of LMW components. Our approach was to work out a suitable method for analysing small peptides in bovine milk and colostrum without extensive sample pre-treatment. Nanostructure-Assisted Laser Desorption/Ionization (NALDI) is a matrix-free method to identify such LMW components. We also made a comparison between MALDI and NALDI for detection of peptides from colostrum samples. Our results show that NALDI provides better intensity compared with MALDI. It allows us to sequence small peptides and to identify a fragment of β-casein from the colostrum sample. Further studies are needed for comprehensive identification and characterization of LMW bioactive peptides from colostrum and milk.

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