Estimation of Particle Size Distribution in Bovine Colostrum Whey by Dynamic Light Scattering (DLS) Method
Estonian University of Life Sciences, Institute of Veterinary Medicine and Animal Sciences, Department of Food Science and Technology, Kreutzwaldi ⁵⁶/⁵, EE⁵¹0¹⁴ Tartu, Estonia; *Correspondence: firstname.lastname@example.org
Colostrum whey consist bioactive compounds in considerable concentration. For isolation of these compounds the particle size has crucial importance. The aim of this work was to study possibilities of using dynamic light scattering method – DLS (Malvern Zetasizer Nano ZS) for colostrum whey particle size distribution estimation. The first and second milking colostrum samples were skimmed by centrifugal separation and casein of these was enzymatically coagulated by rennet (chymosin). Obtained whey was diluted (¹:²00) by distilled water and filtered (cut-off 0.⁴⁵ m) to get probes for estimation of particle size. Particle size distribution in colostrum whey probes had maximally three peaks and polydispersity indices from 0.¹⁵⁷ to 0.⁵⁴¹. Prevailing peak of the distribution was found at size from ¹⁴⁴ to ²¹0 nm, which apparently corresponds to hydrodynamic diameter of immunoglobulin IgG¹.