Tag Archives: iron

1351-1359 B. Dalecka and L. Mezule
Study of potential PCR inhibitors in drinking water for Escherichia coli identification
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Study of potential PCR inhibitors in drinking water for Escherichia coli identification

B. Dalecka* and L. Mezule

Riga Technical University, Faculty of Civil Engineering, Water Research laboratory, Kipsalas 6A-263, LV-1048 Riga, Latvia
*Correspondence: brigita.dalecka_1@rtu.lv

Abstract:

In the last few decades, the polymerase chain reaction (PCR) has become one of the most powerful molecular biological tools. However, the PCR is an enzymatic reaction and therefore sensitive to inhibitors which may occur in drinking water samples. In this work, the possible inhibition effect of chlorine, humic acids, and iron for real-time PCR (qPCR) efficiency was studied and the environmental sample from drinking water treatment system before iron removal was selected and analysed. The results demonstrated that the highest concentrations of humic acids (5 mg L-1 and 1 mg L-1) and iron (4 mg L-1) inhibited the PCR reaction while no effect of chlorine was observed. The analysis of the environmental sample with spiked Escherichia coli cells demonstrated reduction efficiency of the average threshold cycle (Ct) values compared with control dilution series determining the possible inhibition for qPCR assay.

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509-516 M. Gibczyńska, E. Meller, S. Stankowski and Cz. Wołoszyk
Metal content in soil fertilized with brown coal fly ash
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Metal content in soil fertilized with brown coal fly ash

M. Gibczyńska¹, E. Meller², S. Stankowski³ and Cz. Wołoszyk⁴

¹ General Chemistry Department, University of Agriculture, 71 434 Szczecin,ul Słowackiego 17, Poland; e-mail: margi@px.pl
² Department of Soil Science, University of Agriculture, 71 434 Szczecin, ul Słowackiego 17,Poland; e-mail: Edward.Meller@agro.ar.szczecin.pl
³ Soil, Plant Cultivation and Biometry Department, University of Agriculture, 71 434 Szczecin,ul Słowackiego 17, Poland; e-mail: mail sstankowski@hoga.pl
⁴ Department of Environmental Chemistry, University of Agriculture, 71 434 Szczecin,ul Słowackiego 17, Poland; e-mail: woloszyk@agro.ar.szczecin.pl

Abstract:

The aim of the studies was evaluation of brown coal ash produced by Power Plant Group Pątnów-Adamów-Konin for agricultural purposes. The subject matter was the estimation of the influence of fly ash applied to light soil on the metal content of soils (aluminium, iron, manganese, copper, nickel and cobalt). The field experiment was conducted on light soil at the area of the Agricultural Experimental Station in Lipnik, Poland. The experiment was set up by means of randomized complete blocks in 4 replications. The study consisted of testing 7 variants (controlburned lime (CaO) dolomite lime CaCO3⋅MgCO3, ash from 1st electrofilterzone, from 2nd electrofilter zone, from 3rd electrofilter zone and mixture of ashes from three electrofilter zones). Lime fertilizers and ashes were applied in a dose corresponding to 1.0 hydrolytic soil acidity expressed in cmol H+ kg-1 of soil. Brown coal fly ash produced by Power Plants ZE PAK S.A., used as soil fertilizer, did not contribute to changes of content of the following metals: iron, zinc, copper, nickel, lead and cobalt in the tested soil. Soil-incorporated brown coal fly ash significantly increased the content of manganese. However, the obtained results did not exceed the allowable standard. In the soil of all fertilizer variants, where brown coal ash was applied, the content of mobile aluminium diminished. When brown coal ash produced by Power Plants ZE PAK S.A. was applied in the experiment, soil reaction changed from acid to neutral and its hydrolytic acidity decreased by ca 50%.

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