Tag Archives: oxidative stress

xxx V. Karpenko, S. Pavlyshyn, R. Prytulіak and D. Naherniuk
Content of malondialdehyde and activity of enzyme glutathione-S-transferase in the leaves of emmer wheat under the action of herbicide and plant growth regulator
Abstract |
Full text PDF (593 kB)

Content of malondialdehyde and activity of enzyme glutathione-S-transferase in the leaves of emmer wheat under the action of herbicide and plant growth regulator

V. Karpenko¹*, S. Pavlyshyn¹, R. Prytulіak¹ and D. Naherniuk²

¹Uman National University of Horticulture, Faculty of Horticulture, Ecology and Plants Protection, Department of Biology, Instytutska street, 1, UA20300 Uman, Ukraine
²Uman National University of Horticulture, Faculty of Management, Department of Management, Instytutska street, 1, UA20300 Uman, Ukraine
*Correspondence: unuh1844@gmail.com

Abstract:

The article presents the results of vegetation experiment on the influence of different rates of herbicide Prima Forte 195 – 2-ethylhexyl ether 2.4-D + aminopyralid + florasulam (0.5; 0.6 and 0.7 L ha-1) under different application methods of plant growth regulator of a natural origin Wuxal BIO Vita (Ascophyllum nodosum extract + microelements) on the content of malondialdehyde (MDA) as an important indicator of the intensity of peroxide oxidation of lipids in plants and on the activity of enzyme glutathione-S-transferase (GST) in the leaves of emmer wheat.
Experimental scheme included 16 experimental variants: 1 – without application of preparations and pre-sowing treatment of seeds (control); 2, 3, 4 – Prima Forte 195, applied to vegetative plants at the rates 0.5, 0.6 and 0.7 L ha-1; 5 – Wuxal BIO Vita at the rate of 1.0 L ha-1, applied to vegetative plants; 6, 7, 8 – Prima Forte 195 at the rates of 0.5, 0.6 and 0.7 L ha-1 in tank mixtures with Wuxal BIO Vita at the rate of 1.0 L ha-1, sprayed on vegetative plants; 9 – pre-sowing treatment of seeds with Wuxal BIO Vita at the rate of 1.0 L t-1 (background); 10, 11, 12 – Prima forte 195, applied to vegetative plants at the rates of 0.5, 0.6 and 0.7 L ha-1 at the background of pre-sowing treatment of seeds with Wuxal BIO Vita at the rate of 1.0 L t-1; 13 – Wuxal BIO Vita at the rate of 1.0 L ha-1 (applied to vegetative plants, treated before sowing with Wuxal BIO Vita at the rate of 1.0 L t-1); 14, 15, 16 – Prima Forte 195 at the rates of 0.5, 0.6 and 0.7 L ha-1 respectively, in tank mixture with Wuxal BІО Vita at the rate of 1.0 L ha -1 – spraying of plants and pre-sowing treatment of seeds with Wuxal BІО Vita 1.0 L t-1.
It has been found that under the application of Prima Forte 195 the redox state in the leaves of emmer wheat increased considerably in the direction of increasing the content of MDA. It has also been proved that herbicide Prima Forte 195 and its mixtures with plant growth regulator (PGR) Wuxal BIO Vita caused the changes in the activity of enzyme glutathione-S-transferase, which slightly increased in the initial period in the variants of integrated application of herbicide and plant growth regulator. However, later the GST activity in the variants of integrated application of herbicide and PGR decreased, which indicates stabilization and a positive influence of integrated application of preparations on the detoxification processes in the crops of emmer wheat.

Key words:

, , , , ,




1517-1526 S. Vasiljeva, G. Smirnova, N. Basova and D. Babarykin
Cadmium-induced oxidative damage and protective action of fractioned red beet (Beta vulgaris) root juice in chickens
Abstract |
Full text PDF (584 kB)

Cadmium-induced oxidative damage and protective action of fractioned red beet (Beta vulgaris) root juice in chickens

S. Vasiljeva¹*, G. Smirnova¹², N. Basova¹ and D. Babarykin²

¹University of Latvia, Institute of Biology, 3 Miera Str., LV-2169 Salaspils, Latvia
²Institute of Innovative Biomedical Technology Ltd, 2 Inchukalna Str., LV-1014 Riga, Latvia
*Correspondence: svetlana.vasiljeva@lu.lv

Abstract:

Cadmium (Cd) is one of the most dangerous environmental bioaccumulative pollutants that affects many organs in humans and animals. Present investigation was conducted to evaluate the protective effect of fractioned red beetroot juice on Cd-induced oxidative stress in chickens. The named red beetroot juice fraction (BJF) was received by juice ultrafiltration. Per oral administration of BJF for 10 days followed by dietary Cd exposure (50 mg kg-1 of diet) was evaluated in the in vivo experiments in chickens. The prominent increase of Cd concentration in blood plasma, liver and kidney provoked the rise of oxidative processes activity in organs. BJF treatment attenuated the Cd-induced oxidative stress. The changes of oxidative stress markers – the reduction of hepatic and kidney malondialdehyde amount, the increase of glutathionperoxidase level in liver and blood catalase activity indicated the possible antioxidative influence of BJF. Chickens exposed to Cd showed no evidence of clinical toxicity, but exhibited some features of adverse action of this heavy metal. The increase of uric acid concentration in blood serum is associated with protein catabolic processes intensified by Cd affect. Suppressive effect of Cd on the immune response in chickens manifested in alteration of cell and humoral immunity parameters. The data of the most investigated oxidative stress markers, biochemical and immunological indices in Cd-exposed chickens were almost back to the values, when received BJF per os during 10 days. Administration of fractioned red beetroot juice to Cd-treated chickens prevented the oxidative impact of this heavy metal and provided immunomodulating effect.

Key words:

, , , ,