Tag Archives: somatic cell count

144–154 G. Gulbe, S. Doniņa, V. Saulīte, J. Jermolajevs, L. Peškova, Š. Svirskis and A. Valdovska
The effect of immunomodulation composition on systemic immune response and udder health in case of bovine subclinical mastitis
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The effect of immunomodulation composition on systemic immune response and udder health in case of bovine subclinical mastitis

G. Gulbe¹*, S. Doniņa¹, V. Saulīte¹, J. Jermolajevs¹, L. Peškova¹, Š. Svirskis¹ and A. Valdovska¹²

¹Riga Stradins University, A. Kirchenstein Institute of Microbiology and Virology, Ratsupites street 5, Riga, LV-1067, Latvia
²Latvia University of Agriculture, Faculty of Veterinary medicine, Institute of Food and Environmental Hygiene, Kr. Helmana street 8, Riga, LV-3004, Latvia
*Correspondence: Gundega.Gulbe85@gmail.com

Abstract:

Modulation of mammary gland immune response may offer an alternative to antimicrobial therapy in the treatment of subclinical mastitis. The aim of the study is to investigate the systemic immunomodulating effect and the impact of composition LLG which consists of lysozyme, lactic acid and glycopeptides isolated from Lactobacillus spp. on udder health parameters. A total of 10 cows with subclinical mastitis were used in the study – 5 cows (19 udder quarters) in the experimental group and 5 other cows (19 udder quarters) in the control group. The experimental cows received intramammary infusions of LLG, given once per day, 3 times with the intervals of 48 h. Cows from the control group received infusions of sterile 0.15 M NaCl, given in the same way as the treated cows. The following parameters were measured: somatic cell count (SCC), the total number of blood leucocyte, differentiation between banded neutrophils, segmented neutrophils and lymphocytes, peripheral blood mononuclear cells and markers of cell activation. Besides bacteriological culturing was performed. Quarter milk and blood samples were taken several times from the 1st to the 21st day. During the treatment the number of lymphocytes and T helper cells significantly decreased in the blood of the treated group, the CD8+ cells did not change remarkably, the number of CD25+, CD38+, as well as CD69+ and CD95+ cells had diminished during the treatment. On the 21st day a rapid increase of IL-2 receptor bearing cells was detected. A significant elevation of SCC in the treated group was observed but pathogenic bacteria incidence decreased.

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1396–1406 T. Michlová, H. Dragounová, R. Seydlová and A. Hejtmánková
The hygienic and nutritional quality of milk from Saanen goats bred in the Moravian-Silesian region
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The hygienic and nutritional quality of milk from Saanen goats bred in the Moravian-Silesian region

T. Michlová¹*, H. Dragounová², R. Seydlová² and A. Hejtmánková¹

¹Czech University of Life Sciences Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Chemistry, Kamýcká 129, CZ165 21, Prague, Czech Republic
²Dairy Research Institute Ltd, Ke dvoru 791/12A, CZ160 00, Prague, Czech Republic
*Correspondence: michlova@af.czu.cz

Abstract:

 The aim of the study was to monitor milk yield and the hygienic and nutritional quality of milk of Saanen goats in the Moravian-Silesian region in Czech Republic. Milk samples were collected once a month during the lactation period. The average milk yield in the standardized lactation was 1,100 liters. The somatic cell count in pool samples ranged from 470 x 103 to 696 x 103. The total microorganism count ranged from 3.6 x 103 to 1.4 x 105. The pathogen Staphylococcus aureus was proven no more than in 6.3%. The highest values of all main components of milk were achieved within a relatively short time after kidding (April 2015). The average content of fat was 3.64  0.52 g 100 ml-1, 3.17  0.16 g 100 ml-1 of protein, 2.60  0.06 g 100 ml-1 of casein, 4.56  0.24 g 100 ml-1 of lactose, and 12.02  0.80 g 100 ml-1 of solids. Average content of vitamin A was 0.27  0.14 mg kg-1 and average content of vitamin E was 0.60  0.34 mg kg-1. Content of vitamin E increased almost continuously during the lactation, and the content of vitamin A was significantly higher at the end of lactation. In lyophilized milk powder the average trace metal contents were 7.76  0.92 g kg-1 Ca, 1.62  0.26 g kg-1 Mg, 15.3  1.43 g kg-1 K, 789  111 mg kg-1 Na, 23.2  2.73 mg kg-1 Zn, and 0.85  0.55 mg kg-1 Cu. Contents of minerals varied during the lactation period, but no significant trends were observed.

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