Tag Archives: soybean

2217–2225 M. Zargar, E. Romanova, A. Trifonova, E. Shmelkova and P. Kezimana
AFLP-analysis of genetic diversity in soybean [Glycine max (l.) Merr.] cultivars Russian and foreign selection
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AFLP-analysis of genetic diversity in soybean [Glycine max (l.) Merr.] cultivars Russian and foreign selection

M. Zargar¹*, E. Romanova¹, A. Trifonova², E. Shmelkova¹ and P. Kezimana¹

¹Department of AgroBiotechnology, Institute of Agriculture, RUDN University, RU117198 Moscow, Russia
²Laboratory of Plant Genetics, Vavilov Institute of General Genetics, Russian Academy of Sciences, RU119333 Moscow, Russia
*Correspondence: zargar_m@pfur.ru

Abstract:

Genetic diversity of 11 cultivars of soybean (Glycine max) from a VIR (N.I. Vavilov Institute of Plant Genetic Resources) collection was analyzed by the AFLP (amplified fragment length polymorphism) technique. From 18 tested primer combinations, both of them were selected for further analysis. From these two primer combinations (E32/M60 and E32/M59), 90 DNA fragments were obtained, 39 (43%) of them were polymorphic. Unique DNA fragments were found in accessions SibNIISHoz 6, Soer-4, Determinant. The Jaccard’s similarity indices varied from 0.79 (between Sonata and Ugra) to 0.94 (between Ugra and Garmoniya) with an average of 0.775. Cluster analysis placed the cultivars into the several groups but separation in groups was not related to their origin or biological characteristics, even though cultivars Nordik and Soer-4 (cultivars recommended for Central Black Earth region of Russia) clustered together on the dendrogram. The obtained high values of Jaccard genetic similarity index and low level of polymorphism and differentiation illustrated a relatively low genetic diversity in our studied cultivars, which correlated with different other studies on soybean genetic diversity with AFLP-analysis.

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1419–1426 A.C. Olcay, G. Bilir, Ö. Taş, M. Deniz and D. Ekinci
Partial Purification of β–glucosidase enzyme from soybean (Glycine max) and determination of inhibitory effects two quercetin derivatives on enzyme activity
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Partial Purification of β–glucosidase enzyme from soybean (Glycine max) and determination of inhibitory effects two quercetin derivatives on enzyme activity

A.C. Olcay, G. Bilir, Ö. Taş, M. Deniz and D. Ekinci*

Ondokuz Mayıs University, Faculty of Agriculture, Department of Agricultural Biotechnology, TR 55139 Samsun, Turkey; e-mail: deniz.ekinci@omu.edu.tr

Abstract:

 Glucosidases are enzymes that catalyze the hydrolysis of the glycosidic linkage of glycosides, leading to the formation of a sugar hemiacetal or hemiketal and the corresponding free aglycon. Activity of glucosidases is crucial for several biochemical processes. Thus, discovery of new glucosidase inhibitors is crucially important owing to potential therapeutic applications of this enzyme in the treatment of diabetes, human immunodeficiency virus infection, metastatic cancer, lysosomal storage disease etc. In the current study, inhibitory potential of ‘quercetin’ and its isomeric form ’morin hydrate’ on the activity of β-glucosidase enzyme, present in the extract of soybean (Glycine max L.) seeds, were investigated. The compounds exhibited moderate inhibitory action in low milimolar concentrations. I50 values were calculated as 0.188 and 0.138 mM for quercetin and morin hydrate, respectively. The results have confirmed that these compounds can be used as leads for designations of novel glucosidase inhibitors which would be used in medicinal biotechnology and food science and technology.

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