In vivo evaluation of antioxydant potential and antihyperglycemic effect of Stevia rebaudiana Bertoni
¹University of Saad Dahlab, Faculty of natural and life sciences, Institute of Science and Applied Technology, Department of Food Science, DZ09000 Blida, Algeria
²National Higher School of Agronomy, Food Technology and Human Nutrition Laboratory, Food Technology Department, DZ16000, El Harrach, Algiers, Algeria
³University of Bejaia, Research Laboratory of Biomathematics, Biochemistry, Biophysics and Scientometrics, DZ06000, Bejaia, Algeria
*Correspondence: aissaoui_ourida@univ-blida.dz
Abstract:
Stevia rebaudiana Bertoni (SR) has a high concentration of phytochemicals that promote health and well-being in conditions such as diabetes. This study aims to assess the antihyperglycemic, antioxidant, and antihyperlipidemic effects of SR on diabetes in male rats caused by Alloxan. Forty adult male rats were divided into five groups. For 28 days, SR was administered by gavage. Spinreact and ELISA kits were used to detect serum levels of blood glucose, insulin, liver function, lipid profiles, antioxidant enzymes, and lipid peroxidation. Histopathology was also investigated. Stevia’s free radical scavenging capabilities have an IC50 value of between 34.49 and 39.66 mg L-1. Stevia therapy reduced biochemical markers in diabetic rats (DR). After 28 days, SR raised fasting blood glucose, insulin, and lipid peroxidation serum levels by 52%, 40%, and 27%, respectively. Also, in DR treated with SR, there was a substantial increase in high-density lipoprotein cholesterol (35.8%), superoxide dismutase (30%), total antioxidant status (20%), glutathione peroxidase and reductase. SR improves DR’s pancreas and liver function by enhancing the endogenous antioxidant system. These findings revealed that SR counteracted Alloxan’s necrotic effects by reducing insulin resistance in DR, hence revitalizing pancreatic β–cells.
Key words:
glycemia, HOMA-IR, liver, oxidative stress, pancreas, Stevia