The laboratory estimation of essential oils as biological methods against black cherry aphids M. cerasi
¹Plant protection Department, Institute of Agronomical Sciences, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture, Tr. A. Hlinku, 2, SK94901 Nitra, Slovakia
²Agriculture, Soil Science and Agrochemistry Department, Agronomy and Forestry Faculty, Vinnytsia National Agrarian University, Sonyacha Str., 3, UA21000 Vinnytsia, Ukraine
*Correspondence: matsera.olga.vnau@gmail.com
Abstract:
Plants’ essential oils play a considerable role in the plant integrated protection systems against harmful insects. Therefore the aim of our study was to find the potential repellency and toxicity effects of essential oils (EOs) from Allium sativum L., Ocimum basilicum L., Lavandula angustifolia Mill, Eucalyptus globulus, Labill and Curcuma longa L., and two chemical substances DEET (N, N-diethyl-3-methylbenzamide) and 2-Undecanone on the black cherry aphids Myzus cerasi. Repellent peculiarities were evaluated at five different concentrations: 0.3%, 0.6%, 0.9%, 1.2% and 1.5%, with 4 replications at the interim of 15 and 30 minutes for an olfactometer test and mortality was evaluated after 2, 6 and 24 hours for insecticidal activity test. The repellent index was most influenced by the concentration of essential oil; the percentage of influence was 64%. At the same time, the highest repellency index 95% was achieved at a concentration of 1.5% when using essential oils of A. sativum and E. globulus, as well as when using 2-Undecanone within 30 minutes of waiting. A contact toxicity (mortality) test in Petri dishes showed efficacy for all tested oils and chemicals after a 6-hour treatment period. The lowest concentration that ensured 100% insect mortality was 0.9% when using E. globulus essential oil. The lowest lethal concentration (LC50) value of 0.00240mL/10 mL was obtained with O. basilicum oil and the lowest LC98 value of 0.28209mL/10 mL was obtained with E. globulus oil after a 6-hour treatment period.
Key words:
Myzus cerasi, Olfactometer, plant protection, repellent peculiarities, toxicity