Cultivation of sweet cherry (Cerasus avium (L.) Moench) seedlings with using of phosphate-mobilizing microorganisms

O. Klymenko¹, M. Klymenko¹, L. Kartyzhova² and Z. Alechenkova²

¹ State Nikitsky Botanical Gardens, National Centre of Science, Nikita, Yalta, Ukraine,e-mail: olga.gnbs@mail.ru
² Institute of Microbiology, Academy of Science of Belarus, Minsk, Belarus,e-mail: kartyzhova@mail.ru

Abstract:

In a field experiment the influence of phosphate-mobilizing bacteria (PMB) on growth, development and output of one-year-old grafted sweet cherry seedlings have been studied. Two various phosphate-mobilizing bacteria PMB 6, PMB 7 and their mix were applied for increase of nursery efficiency. We established that the application of PMB increased seedlings thriving, stimulated growth processes, improved frost and winter resistance and vitality of sweet cherry buds, budded on a Cerasus mahaleb. PMB 7 and mix PMB 6 + PMB 7 increased an output of seedlings, reduced the cost price of a planting material and increased the level of profitability. The greatest economic benefit was received by using of the mix PMB 6 + PMB 7.

Key words:

Use of RAPD and SCAR markers for identification of strawberry genotypes carrying red stele (Phytophtora fragariae) resistance gene Rpf1

R. Rugienius*, T. Siksnianas, V. Stanys, D. Gelvonauskiene and V. Bendokas

Lithuanian Institute of Horticulture, Babtai LT-54333, Kaunas distr. Lithuania
*Corresponding author; e-mail: r.rugienius@lsdi.lt

Abstract:

Phytopthora fragariae is responsible for strawberry red stele disease that spreads in almost all areas where strawberries are grown. RAPD markers that were developed previously for the strawberry Rpf1 gene are difficult to reproduce and preferentially, therefore, should be converted into sequence characterized amplified region (SCAR) markers. The aim of our study was to develop new SCAR markers, associated with Rpf1, to make a comparative study of RAPD and SCAR markers, and to select strawberry varieties and seedlings carrying the Rpf1 gene. A SCAR marker was constructed based upon RAPD marker OPO-16C, linked to the susceptibility allele of the Rpf1 gene. Following the cloning and sequencing of this marker we were able to design SCAR primers specific to this gene. Utilizing newly developed SCAR markers it was shown that resistant varieties like ‘Anapolis’, ‘Redgauntlet’ contain Rpf1 region. Common varieties ‘Elsanta’, ‘Kama’, ‘Venta’, ‘Senga Sengana’ did not exhibit this gene. The segregation rate of seedling populations (susceptible x resistant) by this marker was approximately 1: 1.

Key words:

Fragaria ananassa Duch., marker-assisted selection, seedlings, strawberry varieties, wilt disease