Comparison of in vitro regeneration and transformation efficiency among five vietnamese indica rice cultivars

L.Q. Cao¹†, P.D. Nguyen¹, X.H. Pham¹, V.H. La², X.V. Nguyen², T.D. Nguyen²*

¹Department of Molecular Pathology, Institute of Agricultural Genetics, Vietnam Academy of Agricultural Sciences, Hanoi 11956, Vietnam
²Institute of Biotechnology and Food technology, Thai Nguyen University of Agriculture and Forestry, Thai Nguyen 24153, Vietnam
*Correspondence: dungnt@tuaf.edu.vn

Abstract:

This study optimized the in vitro regeneration and transformation processes for five popular indica rice cultivars in Vietnam named as BC15, Bac Thom 7 (BT7), Khang Dan 18 (KD18), OM5451, and TBR225. The highest callus induction rates (83-93.67%) were achieved on a medium containing 2.5 mgL^-l 2,4-D, supplemented with 500 mg L^-l each of L-proline, L-glutamine, and casein. Higher or lower concentrations of 2,4-D resulted in reduced callus induction. Among tested cultivars, BT7 exhibited the highest callus induction rate of 93.67%. Shoot regeneration was optimized on a medium with a combination of 0.5 mg L^-l kinetin, 2.0 mg L^-l BAP, and 0.5 mg L^-l NAA, resulting in regeneration rates ranging from 80.23% to 90.77% across cultivars, with BT7 exhibiting the highest regeneration rate (90.77%). Hygromycin at 20 mg L^-l was optimal for selecting transgenic calli, with BT7 showing the highest transformation efficiency opdetermined to be optimal for selecting transgenic calli, with BT7 achieving the highest transformation efficiency (44% hygromycin-resistant callus and 29% GUS expression). PCR analysis confirmed a high proportion (87.5%) of transgenic plants in the BT7 cultivar. These findings indicate that the BT7 is the most suitable cultivar for genetic transformation and gene editing applications.

Key words:

callus, in vitro, indica rice, regeneration, shoot

The effect of the 1,2,3-triazolo[5,1-b][1,3,4]thiadiazines on Solanum lycopersicum L. seed germination

O.A. Vysokova¹, T.A. Kalinina¹, T.V. Glukhareva¹²*, A.A. Kochubei³ and O.A. Cherepanova³

¹Ural Federal University, Mira street 19, RU620002 Ekaterinburg, Russia
²Postovsky Institute of Organic Synthesis, Ural Branch of the Russian Academy of Sciences, Sofia Kovalevskaya street 22, RU 620990, Ekaterinburg, Russia
³Botanical Garden UB RAS, 8 Marta street 202a, RU620144 Ekaterinburg, Russia
*Correspondence: taniagluhareva@yandex.ru

Abstract:

In recent years chemical pesticides are being replaced by environmentally friendly and universal means of plant protection that are able to exert a complex effect on the plant (stimulate growth and development, improve metabolic processes, develop resistance to pathogens, etc.). The effect of new synthetic growth regulators of the 1,2,3-triazolo[5,1-b][1,3,4]thiadiazine class and trade phytohormones (6-BAP, GA and TDZ) on the growth processes, growth energy and vitality of tomato seeds, as well as morphological parameters of seedlings was researched in this article. It was revealed that the effect of synthesized compounds on seed vigor and viability of seedlings are superior to commercial phytohormones. In the early stages of germination the seed vigor of tomato seeds treated with the compounds TT1-TT5 were superior to one in treatments with phytohormones and in control experiment. As a result of the experiment, the most viable seedlings were formed into the treatments TT1, TT2 and TT3 (in all studied concentrations). Tomato seedlings treated with 6-BAP and GA at a concentration of 5 mg L^-1 produced the worst results.

Key words: