Detection of changes in the defence factors of Nicotiana Tabacum plant under the influence of insertion and expression of heterologous transgenes (desA, desC, HuINFα-2b)
¹Institute of Cell Biology and Genetic Engineering of NAS of Ukraine, Academika Zabolotnoho Str.148, UA03143 Kyiv, Ukraine
²Institute of Microbiology and Virology named after D.K. Zabolotny, Str. Academika Zabolotnoho, 154, UA03143 Kyiv, Ukraine
*Correspondence: t-kirpa@ukr.net
Abstract:
Genetically modified plants may have some changes in physiological and biochemical reactions depending on the type of transgene. In this study, we present the results of the analysis of tobacco plants with the insertion and expression of the genes for human interferon alpha (HuINFα-2b), Δ12-acyl-lipid desaturase (desA of the cyanobacterium Synechocystis sp. PCC 6803) and Δ9-acyl-lipid desaturase (desC of the cyanobacterium Synechococcus vulcanus). Wild-type tobacco plants were used as a control. The level of accumulation of polyfructans and changes in the fatty acid spectrum in the leaves of plants under normal physiological conditions and after exposure to low temperatures were tested. It was found that all transgenic plants had some changes in the composition of fatty acids, however, only plants with the HuINFα-2b gene insertion had an increased content of polyfructans. These data may indirectly indicate a difference in the two defense strategies of the plant organism depending on the insertion and expression of the transferred gene.
Key words:
abiotic stress, acyl-lipid desaturases, adaptation, fatty acids, polyfructans