Tag Archives: hyperhydricity

xxx C.G. Comar, M. dos S. Queiroz, M.M. de Andrade, J.R. Trettel and H.M. Magalhães
Copper modulates the biochemical and enzymatic activity and growth of tomato cultivars grown in vitro
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Copper modulates the biochemical and enzymatic activity and growth of tomato cultivars grown in vitro

C.G. Comar¹, M. dos S. Queiroz², M.M. de Andrade², J.R. Trettel¹ and H.M. Magalhães¹*

¹Paranaense University – UNIPAR, Graduate Program in Biotechnology Applied to Agriculture, 87502-210, Umuarama, Paraná, Brazil
²Paranaense University – UNIPAR, Agronomy, 87502-210, Umuarama, Paraná, Brazil
*Correspondence: helidamara@prof.unipar.br

Abstract:

Copper (Cu) is a micronutrient that is neglected for tomato growth. This study sought to identify the effects of exposure to Cu on the growth and biochemical activity of two tomato cultivars. Tomato seeds of ‘Carolina’ and ‘Cereja’ cultivars were disinfected and inoculated in MS medium plus copper sulfate concentrations (CuSO4) (default MS, 25, 50, and 100 μm) and had their growth monitored for 30 days. It was estimated that the growth and biomass accumulation of tomato plants ‘Carolina’ and ‘Cereja’, both from the aerial part and the roots, were benefited by 25 e 50 μm of CuSO4. However, it was observed that these concentrations were inefficient in controlling hyperhydricity and leaf deformation. There was a reduction of these phenomena in the treatment with 100 μm, in both cultivars. Tomato of ‘Carolina’ cultivar subjected to 100 μm showed an increase in anthocyanins and superoxide dismutase (SOD) activity in the root system. There was a reduction of catalase (CAT) activity in shoots exposed to Cu. ‘Cereja’ tomatoes subjected to 100 μm showed an increase in CAT and SOD activity in shoots and roots, respectively. It was concluded that the ‘Carolina’ and ‘Cereja’ tomatoes have their growth impaired when exposed to 100 μm CuSO4. Concentrations higher than 50 μm of CuSO4 cause an increase in the antioxidant activity in the shoot of tomato plants from the ‘Carolina’ cultivar. Concentrations higher than 50 μm CuSO4 increase SOD activity in the root system of tomato plants from the ‘Cereja’ cultivar.

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603–618 J.R. Trettel, M. D.S. Queiroz, M.M. Andrade and H.M. Magalhães
In vitro effects of regulators on growth and morphogenesis of Ocimum basilicum L. ‘Alfavaca Green’ stem apexes
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In vitro effects of regulators on growth and morphogenesis of Ocimum basilicum L. ‘Alfavaca Green’ stem apexes

J.R. Trettel, M. D.S. Queiroz, M.M. Andrade and H.M. Magalhães*

University Paranaense, Department of Biotechnology, Mascarenhas de Moraes Square, 4282, BR87502-210 Umuarama, Paraná, Brazil
*Correspondence: helidamara@prof.unipar.br

Abstract:

Large-scale cultivation of contamination free plants requires a good standardization protocol and production methods. Basil is widely used for cosmetics, food and pharmaceutical industries as it is rich in many bioactive compounds. This present study aimed to evaluate the growth and in vitro anatomical aspects of apical buds of basil grown under different concentrations rowth regulators like: NAA (Naphthalenoacetic Acid), BAP (6-benzylaminopurine), and KIN (Kinetin). The in vitro establishment was evaluated every 20 days to calculate the, the percentage of plants with calluses, appearance of the roots, any abnormal seedlings, any oxidized seedlings, and the number of sprouts per plant. Growth, physiological, and morpho-anatomical evaluations were performed at 80 days. Basal callogenesis was observed when cytokinin’s and auxins are used in combination. Auxin treatments caused hyperhydricity in the stems and leaves. Medium A2 (0.05 mg L-1 of NAA and 0.1 mg L-1 of BAP), and A3 (0.05 mg L-1 of NAA and 0.1 mg L-1 of KIN) resulted in the best development of basil plants, cultivar ‘Alfavaca Green’. The A2 produced plants with greater numbers of leaves, an average bud length of 59.81 mm, and the best root properties. A2 and A1 have a higher percentage of hyperhydricity (83 and 67%). The A3 resulted in an acceptable number of leaves (range: 21–39), and this treatment produced the best shoot properties as well as fewer plants with hyperhydricity. In addition, the A3 treatment produced plants with a shoot length, high shoot fresh and dry mass (2.82 and 0.23 g), high chlorophyll index and leaf anatomy that was similar to the control. Excluding the control, the other treatments presented more than 90% of the explants with calluses in their bases.

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