Tag Archives: PCR analysis

531-548 E. Dubina, P. Kostylev, S. Garkusha, M. Ruban, S. Lesnyak, Y. Makukha, S. Korzh, D. Nartymov and O. Gorun
The use of SSR-markers in rice breeding for resistance to blast and submergence tolerance
Abstract |

The use of SSR-markers in rice breeding for resistance to blast and submergence tolerance

E. Dubina¹²*, P. Kostylev³, S. Garkusha¹², M. Ruban¹, S. Lesnyak¹, Y. Makukha¹, S. Korzh¹, D. Nartymov¹ and O. Gorun¹

¹Federal Scientific Rice Centre, Laboratory of Informational, Digital and Biotechnologies, Belozerny 3, RU350921 Krasnodar, Russia
²Kuban State Agrarian University, Faculty of Agronomy, Department of Biotechnology, Biochemistry and Biophysics, Kalinina 13, RU350004 Krasnodar, Russia
³Agricultural research center ‘Donskoy’, Laboratory of Rice Breeding and Seed Production, Nauchny Gorodok 3, RU347740 Zernograd, Russia
*Correspondence: lenakrug1@rambler.ru


The identification of effective specialized DNA markers providing the clear control of target locus inheritance by the trait of submergence tolerance has been conducted. Among the studied set of microsatellite markers, two the most informative SSR-markers – RM 7481, PrC3 showed high efficiency in detecting intraspecific polymorphism of rice varieties and lines used in the work. With the use of these markers the clear genotype marking the obtained hybrid rice plants by this trait has been conducted and it is has been verified by phenotype evaluation as a result of laboratory trials. The plant samples carrying the target gene in heterozygous and homozygous state has been selected. About 400 backcrossed self-pollinated rice lines with introgressed and pyramided resistance genes Pi-1, Pi-2, Pi-33, Pi-ta, Pi-b to Pyricularia oryzae Cav. were obtained within the frameworks of program to develop genetic rice sources resistant to blast. The conducted testing for resistance to blast and the assessment by economically valuable traits have allowed to select the prospective rice samples. The plant samples of F2 and BC1F1 generations with combination of resistance to blast genes (Pi) and submergence tolerance gene (Sub1A) in homozygous and heterozygous state that is confirmed be the results of analysis of their DNA have been obtained. The obtained hybrid plants are being tested in breeding nurseries for a complex of economically valuable traits. The best plants will be selected and send to State Variety Testing system. Their involving in rice industry will reduce the use of plant protection chemicals against diseases and weeds, thereby increasing the ecology status of the rice industry.

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