Tag Archives: rice

531-548 E. Dubina, P. Kostylev, S. Garkusha, M. Ruban, S. Lesnyak, Y. Makukha, S. Korzh, D. Nartymov and O. Gorun
The use of SSR-markers in rice breeding for resistance to blast and submergence tolerance
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The use of SSR-markers in rice breeding for resistance to blast and submergence tolerance

E. Dubina¹²*, P. Kostylev³, S. Garkusha¹², M. Ruban¹, S. Lesnyak¹, Y. Makukha¹, S. Korzh¹, D. Nartymov¹ and O. Gorun¹

¹Federal Scientific Rice Centre, Laboratory of Informational, Digital and Biotechnologies, Belozerny 3, RU350921 Krasnodar, Russia
²Kuban State Agrarian University, Faculty of Agronomy, Department of Biotechnology, Biochemistry and Biophysics, Kalinina 13, RU350004 Krasnodar, Russia
³Agricultural research center ‘Donskoy’, Laboratory of Rice Breeding and Seed Production, Nauchny Gorodok 3, RU347740 Zernograd, Russia
*Correspondence: lenakrug1@rambler.ru

Abstract:

The identification of effective specialized DNA markers providing the clear control of target locus inheritance by the trait of submergence tolerance has been conducted. Among the studied set of microsatellite markers, two the most informative SSR-markers – RM 7481, PrC3 showed high efficiency in detecting intraspecific polymorphism of rice varieties and lines used in the work. With the use of these markers the clear genotype marking the obtained hybrid rice plants by this trait has been conducted and it is has been verified by phenotype evaluation as a result of laboratory trials. The plant samples carrying the target gene in heterozygous and homozygous state has been selected. About 400 backcrossed self-pollinated rice lines with introgressed and pyramided resistance genes Pi-1, Pi-2, Pi-33, Pi-ta, Pi-b to Pyricularia oryzae Cav. were obtained within the frameworks of program to develop genetic rice sources resistant to blast. The conducted testing for resistance to blast and the assessment by economically valuable traits have allowed to select the prospective rice samples. The plant samples of F2 and BC1F1 generations with combination of resistance to blast genes (Pi) and submergence tolerance gene (Sub1A) in homozygous and heterozygous state that is confirmed be the results of analysis of their DNA have been obtained. The obtained hybrid plants are being tested in breeding nurseries for a complex of economically valuable traits. The best plants will be selected and send to State Variety Testing system. Their involving in rice industry will reduce the use of plant protection chemicals against diseases and weeds, thereby increasing the ecology status of the rice industry.

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284-288 I. Lignicka, A. Graci (Balgalve) and A.M. Zīdere - Laizāne
Amino acid content in rice and lentil meal for vegan and pescatarian diet
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Amino acid content in rice and lentil meal for vegan and pescatarian diet

I. Lignicka*, A. Graci (Balgalve) and A.M. Zīdere - Laizāne

Felici LLC, Rigas gatve 8, LV-2164 Adazi, Adazu novads, Latvia
*Correspondence: ilva.lignicka@musli.lv

Abstract:

Combining different raw materials, it is possible to increase plant-based protein functionality. Traditionally lentils are combined with rice. As rice complements lentils in sulphur-containing amino acids this plant-based combination provides a complete profile of essential amino acids. The aim of this study was to compare amino acid content and scoring pattern in vegan and pescatarian quick preparation meals and analyse developed meal suitability for a vegan diet containing all needed amino acids. Results show that vegan and pescatarian quick preparation meals contain all essential amino acids at adequate amounts according to FAO`s recommendation, results showed no significant difference between samples (p > 0.05). These results show that combining rice and lentils it is possible to develop a meal suitable for a vegan diet that contains all needed amino acids.

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229-234 I. Lignicka, A. Graci (Balgalve) and A.M. Zīdere-Laizāne
Nutritious lentil and rice meal for sustainable vegan and pescatarian diet
Abstract |
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Nutritious lentil and rice meal for sustainable vegan and pescatarian diet

I. Lignicka*, A. Graci (Balgalve) and A.M. Zīdere-Laizāne

Felici LLC, Rigas gatve 8, LV-2164 Adazi, Adazu novads, Latvia
*Correspondence: ilva.lignicka@musli.lv

Abstract:

Urbanization has been accompanied by changes towards diets that have placed increased pressure on the environment and human health. Shifting diet from beef towards less-impactful proteins is the most important policy goal for dietary sustainability in 2050. Fish can also help to shift to lower emission diets, fish-eaters have nearly the same emissions profile as strict vegetarians. The aim of this study was to develop quick preparation lentil and rice meals that would be suitable for a sustainable vegan and pescatarian diet and to analyse and compare their nutritional values. Sample nutritional values were calculated according to raw material nutritional values. Results show that if most raw materials are plant-based there is no significant difference between vegan and pescatarian quick preparation meal sample nutritional values other than minerals and vitamins. Vegan quick preparation meal sample which contains green lentils has higher iron content comparing to pescatarian quick preparation meal which contains red lentils and freeze-dried salmon powder and has higher vitamin B1, B3 and B6 content.

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