Tag Archives: total phenolic content

814-826 L. Shevchuk, Y. Tereshchenko, Y. Vintskovska, L. Levchuk, S. Babenko and R. Hrynyk
Yield and content of biologically active substances in blue honeysuckle fruit (Lonicera caerulea L.) grown in the Forest Steppe of Ukraine
Abstract |
Full text PDF (227 KB)

Yield and content of biologically active substances in blue honeysuckle fruit (Lonicera caerulea L.) grown in the Forest Steppe of Ukraine

L. Shevchuk¹²*, Y. Tereshchenko², Y. Vintskovska², L. Levchuk², S. Babenko² and R. Hrynyk²

¹National University of Life and Environmental Sciences of Ukraine, Agrobiological Faculty, 15 Heroes of Defense Str., UA03041 Kyiv, Ukraine
²Institute of Horticulture of the National Academy of Agrarian Sciences of Ukraine, 23 Sadova Str., UA03027 Kyiv, Ukraine
*Correspondence: zberig@ukr.net

Abstract:

The blue honeysuckle (Lonicera caerulea L.) is a relatively new crop in Ukraine, its industrial cultivation is only 100 hectares. The main constraints are the lack of varieties with high yield and nutritional value of berries. Therefore, a study of the yield and quality of introduced varieties is necessary and relevant, both for producers and breeders. With our research, we determined the potential of the early stage of blue honeysuckle berries under the conditions of their cultivation in the Forest Steppe of Ukraine and the weather conditions of the year of the specified region. We assessed how early we can get a crop and set what quality and what it will be. To clearly understand the quality of the grown fruits, their average weight, size and uniformity were studied. From nutritional indicators of fruit quality, the content of dry matter, soluble solids, sugars and titrated acids was studied, from biologically active substances, the content of vitamin C and total phenolic was determined. It was found that in the zone of the Forest Steppe of Ukraine from the studied group of varieties, the highest yield potential, 3.13 kg from a bush for the second year of fruiting and fruit mass 2.4 g, had a variety of Canadian breeding ‘Boreal Beauty’. The fruits of the cultivars ‘Duet’ (4.3) and ‘Boreal Blizzard’ (4.5) were distinguished by the balance of taste according to the sugar-acid index, and the maximum amount of total phenolics for the studied group of varieties was accumulated by the fruits of ‘Boreal Beast’ (1,000 mg 100 g-1).

Key words:

, , , , ,



1860–1872 A. Ramata-Stunda, V. Valkovska, M. Borodušķis, D. Livkiša, E. Kaktiņa, B. Silamiķele, A. Borodušķe, A. Pentjušs and N. Rostoks
Development of metabolic engineering approaches to regulate the content of total phenolics, antiradical activity and organic acids in callus cultures of the highbush blueberry (Vaccinium corymbosum L.)
Abstract |
Full text PDF (478 KB)

Development of metabolic engineering approaches to regulate the content of total phenolics, antiradical activity and organic acids in callus cultures of the highbush blueberry (Vaccinium corymbosum L.)

A. Ramata-Stunda¹, V. Valkovska², M. Borodušķis¹, D. Livkiša¹, E. Kaktiņa¹, B. Silamiķele¹, A. Borodušķe¹, A. Pentjušs³ and N. Rostoks¹*

¹Faculty of Biology, University of Latvia, Jelgavas street 1, LV-1004 Riga, Latvia
²Faculty of Chemistry, University of Latvia, Jelgavas street 1, LV-1004 Riga, Latvia
³Institute of Microbiology and Biotechnology, University of Latvia, Jelgavas street 1, LV-1004 Riga, Latvia
*Correspondence: nils.rostoks@lu.lv

Abstract:

Blueberry (Vaccinium corymbosum L.) is increasingly cultivated to produce high quality berries for consumption and potential applications in medicine, nutrition and as industrial precursors. Seasonal availability sets limitations on chemical compound isolation from cultivated plants. Biotechnological solutions, such as tissue cultures and metabolic engineering, can provide sufficient amounts of plant material with reasonably high metabolite levels, which may be adjusted by different strategies. Here, we describe our approach to modifying total phenolic content (TPC), antiradical activity (ARA) and amounts of selected organic acids in in vitro cultures of two varieties of V. corymbosum by varying the growth media. TPC, ARA and acid levels were determined in mature leaves of field-grown plants and in stable callus cultures derived from leaves of varieties ‘Bluecrop’ and ‘Duke’ grown on Murashige-Skoog (MS) and Woody plant (WP) media supplemented with varying concentrations and combinations of different plant growth hormones. TPC varied from 83 mg g-1 dry weight (DW) to 142 mg g-1 DW in leaves of ‘Bluecrop’ and ‘Duke’, respectively, and correlated with their ARA with ‘Duke’ at the lead. For callus cultures the highest ARA, as well as the highest TPC of 94 mg g-1 DW was observed in ‘Bluecrop’ grown on WP medium with 2,4-dichlorophenoxyacetic acid (2,4-D). High level of quinic acid was found in the mature leaves of all tested varieties, while callus cultures exhibited relative increase in amounts of malic, succinic and citric acids instead. Oxalic acid was found only in callus cultures.

Key words:

, , , , ,



1425-1434 G. Krumina–Zemture, I. Beitane and I. Cinkmanis
Flavonoids and total phenolic content in extruded buckwheat products with sweet and salty taste
Abstract |
Full text PDF (1 MB)

Flavonoids and total phenolic content in extruded buckwheat products with sweet and salty taste

G. Krumina–Zemture¹*, I. Beitane¹ and I. Cinkmanis²

¹Latvia University of Agriculture, Faculty of Food Technology, Department of Nutrition, Riga street 22, LV–3004, Jelgava, Latvia
²Latvia University of Agriculture, Faculty of Food Technology, Department of Chemistry, Liela street 2, LV–3001, Jelgava, Latvia
*Correspondence: gita.krumina@llu.lv

Abstract:

The aim of research was to evaluate the effect of added ingredients used for taste improvement on flavonoids and total phenolic content in extruded buckwheat products. The added ingredients were – sugar, vanilla sugar, stevia, agave syrup, cinnamon, caraway, garlic powder, sweet pepper powder and salt. Six extruded buckwheat products were analysed, where an extruded buckwheat product without added ingredients was a control sample, two extruded buckwheat products were with sweet taste and three products with salty taste. Total phenolic content was determined using the modified Folin–Ciocalteu method but flavonoid content according to LC-TOF-MS method.
The highest total phenolic content (p < 0.05) was determined in extruded buckwheat product with vanilla sugar + stevia (91.17 ± 0.67 GAE mg 100g-1DW). It was almost three times higher than in the control sample. The significant differences were observed in extruded buckwheat products with caraway + salt + agave syrup (41.5 ± 0.12 GAE mg 100g-1DW) and sweet pepper powder + salt + agave syrup (42.39 ± 0.80 GAE mg 100g-1DW) comparing to other extruded products (p < 0.05). The highest content of rutin and quercetin (p < 0.05) was established in extruded buckwheat product with garlic powder + salt + agave syrup, whereas the highest content of catechin and epicatechin – in extruded buckwheat product with vanilla sugar + stevia. The extruded buckwheat product with caraway + salt + agave syrup in addition contained luteolin, kaempferol and isoquercitrin. The results of research showed that some added ingredients used for taste improvement can significantly influence the total phenolic content and flavonoid content.

Key words:

, , ,



1287–1294 M. Eliášová and L. Paznocht
Total phenolic content and antioxidant activity of tritordeum wheat and barley
Abstract |
Full text PDF (244 KB)

Total phenolic content and antioxidant activity of tritordeum wheat and barley

M. Eliášová* and L. Paznocht

Czech University of Life Sciences Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Chemistry, Kamýcka 129, CZ16521, Prague 6, Czech Republic
*Correspondence: eliasovam@af.czu.cz

Abstract:

Whole grains are a source of numerous antioxidant compounds such as phenolic compounds, anthocyanins, phenolic acids, proanthocyanidins, lignans and others which are able to scavenge free radicals. Thus cereals seem to be very useful in preventing chronic diseases like metabolic syndrome (obesity, high blood pressure, high blood triglyceride and glucose levels), diabetes, neurodegenerative diseases, cancer and chronic inflammatory diseases. Recently, there has been an increased consumer demand for cereal based foods, especially whole cereals. Such demand provides scope for innovations of which an important one is introduction of a completely new cereal cross called tritordeum. This alternative cereal, which is presented as a good source of health beneficial compounds, was assessed in this study and compared with wheat and barley. The total phenolic content (TPC) and related total antioxidant activity (TAA) were investigated via two spectrophotometric methods using a stable 2,2-diphenyl-1-picryhydrazyl radical (DPPH) and Folin-Ciocalteau reagent respectively. Both TPC and related TAA values of tritordeum and wheat were similar but were significantly lower compared to barley. Results have also revealed a close relationship between TAA and TPC (R2 = 0.93, p < 0.05), which might suggest that increased antioxidant activity in those grains is caused by phenolic compounds contained in them. Tritordeum seems to represent a new cereal with good prospects; nevertheless more detailed analysis of its health related compounds is required.

Key words:

, , , , ,