Tag Archives: biopreparations

2436–2448 L.E. Kolesnikov, I.I. Novikova, E.V. Popova, N.S. Priyatkin, E.V. Zuev, Yu.R. Kolesnikova and M.D. Solodyannikov
The effectiveness of biopreparations in soft wheat cultivation and the quality assessment of the grain by the digital x-ray imaging
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The effectiveness of biopreparations in soft wheat cultivation and the quality assessment of the grain by the digital x-ray imaging

L.E. Kolesnikov¹*, I.I. Novikova², E.V. Popova², N.S. Priyatkin³, E.V. Zuev⁴, Yu.R. Kolesnikova⁴ and M.D. Solodyannikov¹

¹Saint-Petersburg State Agrarian University, Faculty of Agrotechnologies, Soil science and Ecology, Department of Plant Protection and Quarantine, Petersburgskoe Shosse (highway), 2, RU196601 St-Petersburg – Pushkin, Russia
²All-Russian Institute of Plant Protection (VIZR), Laboratory of microbiological plant protection, shosse Podbel’skogo highway, 3, RU196608 Saint Petersburg-Pushkin, Russia
³Agrophysical Research Institute (ARI), plant biophysics division, Grazhdanskiy ave. 14, RU195220 St-Petersburg, Russia
⁴Federal Research Center N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR), genetic resource of wheat department, plant introduction department, Bolshaya Morskaya street, 42–44, RU190000 St–Petersburg, Russia
*Correspondence: kleon9@yandex.ru

Abstract:

The main trend of modern crop production is the expansion of the use of plant protection solutions and technologies, that ensure not only effective management of the number of populations of harmful organisms, but also the production of environmentally safe agricultural products with minimal anthropogenic impact on agro-ecosystems. One of our priorities is to develop new environmentally sound polyfunctional biopreparations, that combine useful properties of microbial strains such as phytopathogen antagonists and chitosan compositions that increase disease resistance. The introscopic analysis of the seed material quality realized with the non–destructive express techniques application was used for evaluation the effectiveness of the compositions’ complex influence on plants. The research studies the effectiveness of the influence of Bacillus subtilis strains and chitosan compositions, including their combined effect, on soft wheat productivity and its damage by disease agents. Data on the quality assessment of the grain using microfocus x-ray radiography and gas-discharge visualization (electrophotography) are also provided. The complex of more than 20 structural elements of wheat productivity was analysed during the research. Assessment of the plant damage intensity according to the standard criterion – the disease development, and additional phytopathological indicators was carried out. The evaluation of the grains’ introscopic characteristics was carried out by the microfocus x-ray radiography techniques combined with the digital x-ray image analysis. It is shown that the biopreparation complexes, containing B. subtilis strains and Chitosan, have a combined biological activity manifested in the optimization of wheat plants’ physiological state, increasing productivity, diseases resistance.

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2156-2168 E.N. Shcherbakova, A.V. Shcherbakov, P.Yu. Rots, L.N. Gonchar, S.A. Mulina, L.M. Yahina, Yu.V. Lactionov and V.K. Chebotar
Inoculation technology for legumes based on alginate encapsulation
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Inoculation technology for legumes based on alginate encapsulation

E.N. Shcherbakova¹*, A.V. Shcherbakov¹, P.Yu. Rots², L.N. Gonchar³, S.A. Mulina¹, L.M. Yahina¹, Yu.V. Lactionov¹ and V.K. Chebotar¹

¹All-Russia research Institute for Agricultural Microbiology, Shosse Podbelskogo 3, RU196608 Pushkin, St. Petersburg, Russia
²Biocad Biotechnology Company, Sviazi street 34, Strelna, RU198515 St. Petersburg,
Russia
³National University of Life and Environmental Sciences of Ukraine, Plant Science Department, Heroyiv Oborony street 15, UA03041 Kyiv, Ukraine
*Correspondence: alonagonchar@mail.ru

Abstract:

The main purpose of seeds inoculation is to provide the sufficient number of viable efficient bacteria that are able to actively colonize the plant roots immediately after germination. One of the promising forms of bacterial preparations is cells encapsulation in the polymer gel. Advantages of using alginate microspheres are slow, controlled release of bacteria, biodegradation in the soil and an increased shelf life. As a result of this study the effectiveness of using capsulated biopreparation was established to increase the nitrogen-fixing potential of legumes. The advantage in colonization activity is shown in comparison with other forms of the biopreparations due to the slow release of rhizobium from the capsules. The optimal composition for formulation is established which ensures the storage of biopreparation for more than 1 year. The prospect of using encapsulated biopreparations under adverse environmental conditions and for joint application with chemical pesticides and agrochemicals is analyzed.

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