Some features of cultivating different Chamaenerion angustifolium (L.) Scop. forms in vitro
¹N.V. Tsitsin Main Botanical Garden of Russian Academy of Sciences, Botanicheskaya
street 4, RU127276 Moscow, Russia
²All–Russian Scientific Research Institute of Agricultural Biotechnology, Timiryazevskaya street 42, RU127550 Moscow, Russia
*Correspondence: greenpro2007@rambler.ru
Abstract:
Chamaenerion angustifolium (L.) Scop. characterized by a wide range of economically useful properties. White-flowered form of Ch. angustifolium (L.) Scop. Is extremely rare in nature. At the same time, it is promising as a source of biologically active substances and as a highly decorative plant. The optimal way to reproduce this form is clonal micropropagation. Methods for obtaining Ch. angustifolium in vitro were developed, as well as the optimal selection timing of starting material for micropropagation was determined. In addition, the effect of a mineral composition of nutrient medium and plant growth regulators on the regeneration of microshoots was studied. The highest values of morphometric parameters were achieved on MS medium (Murashige & Skoog, 1962) supplemented with 0.5 mg L-1 BAP. The multiplication factor of the lilac–flowered form was 8.4 ± 0.2, of the white-flowered form – 9.2 ± 0.6. Comparative analysis of morphometric parameters during cultivation of Ch. angustifolium showed no significant difference between the lilac-flowered and white-flowered forms. The effect of antioxidants on the growth and development of regenerants has been shown. The most optimal nutrient medium for clonal micropropagation of the lilac-flowered form was MS medium (Murashige & Skoog, 1962) containing 0.5 mg L-1 of BAP, 50 mg L-1 of ascorbic acid and 50 mg L-1 of citric acids and for micropropagation of the white–flowered form it was the medium, containing 100 mg L-1 PVP.
Key words:
Clonal micropropagation, fireweed, phenolic exudation, plant growth regulators