In vivo evaluation of antioxydant potential and antihyperglycemic effect of Stevia rebaudiana Bertoni

O. Aissaoui¹²*, L. Terki¹³, S. Ait Ameur² and A. Bitam²

¹University of Saad Dahlab, Faculty of natural and life sciences, Institute of Science and Applied Technology, Department of Food Science, DZ09000 Blida, Algeria
²National Higher School of Agronomy, Food Technology and Human Nutrition Laboratory, Food Technology Department, DZ16000, El Harrach, Algiers, Algeria
³University of Bejaia, Research Laboratory of Biomathematics, Biochemistry, Biophysics and Scientometrics, DZ06000, Bejaia, Algeria
*Correspondence: aissaoui_ourida@univ-blida.dz

Abstract:

Stevia rebaudiana Bertoni (SR) has a high concentration of phytochemicals that promote health and well-being in conditions such as diabetes. This study aims to assess the antihyperglycemic, antioxidant, and antihyperlipidemic effects of SR on diabetes in male rats caused by Alloxan. Forty adult male rats were divided into five groups. For 28 days, SR was administered by gavage. Spinreact and ELISA kits were used to detect serum levels of blood glucose, insulin, liver function, lipid profiles, antioxidant enzymes, and lipid peroxidation. Histopathology was also investigated. Stevia’s free radical scavenging capabilities have an IC₅₀ value of between 34.49 and 39.66 mg L^-1. Stevia therapy reduced biochemical markers in diabetic rats (DR). After 28 days, SR raised fasting blood glucose, insulin, and lipid peroxidation serum levels by 52%, 40%, and 27%, respectively. Also, in DR treated with SR, there was a substantial increase in high-density lipoprotein cholesterol (35.8%), superoxide dismutase (30%), total antioxidant status (20%), glutathione peroxidase and reductase. SR improves DR’s pancreas and liver function by enhancing the endogenous antioxidant system. These findings revealed that SR counteracted Alloxan’s necrotic effects by reducing insulin resistance in DR, hence revitalizing pancreatic β–cells.

Key words:

Study of influence of proteolytic action fodder additive in the composition of feed on productivity and development of broilers chickens’ internal organs

E.V. Shatskikh¹,*, O.V. Molokanova¹ and R.Yu. Timoshenko²

¹Ural State Agrarian University, Department of Biotechnology and Food Products,
Street Karl Liebknecht 42, RU620075 Yekaterinburg, Russia
²Rep. office of Novus Europe S.A./N.V., Street Pryanishnikova 23A, of.33 RU127550
Moscow, Russia

Abstract:

In the system of comprehensive assessment of feed nutritional value, protein plays a special role. With the correct selection of proteolytic enzymes in the diet and with appropriate conditions (pH, temperature, duration and quantity of enzymes), most feed proteins can be degraded to preferred degree in the body: either partially, limited by proteolysis, or deep and complete – to individual amino acids, which is accompanied by an increase in animal productivity.
The objective was to study the efficiency of using the exogenous enzyme Sibenza DP 100 in the diet on productivity and development of broilers chickens’ internal organs. The experimental part of the work was carried out under the industrial conditions of private limited company ‘LISKoBroiler’ on Ross 308 broiler chickens. When the proteolytic enzyme was added to the birds’ diet without decreasing the nutritional value of crude protein and digestible aminoacids, higher values of the average daily gain were observed among all experimental groups compared to the control group, while reducing feed output per 1 kg of gain. While reducing the nutritional diet value in accordance with the matrix by 2.5% for crude protein and digestible amino acids and with the addition of the Sibenza DP 100 enzyme (500 g t^-1), growing broilers was characterized by a slight lag in live weight compared to the control group, but with the most effective consumption feed per 1 kg of gain. The abovementioned system for the proteolytic enzyme use had the most positive effect on the morphological structure of the pancreas of broiler chickens.

Key words:

broiler chickens, enzyme preparation, intestinal length., nutrient digestibility, pancreas, protease