Activation effect of β-alanine and chitosan derivative on A. glycyphyllos and A. membranaceus seed germination and seedling growth and development

L.A. Khamidullina¹²*, O.E. Cherepanova³, P.D. Tobysheva², E.A. Rybina² and A.V. Pestov¹²

¹Postovsky Institute of Organic Synthesis, UB RAS, Sofia Kovalevskaya street 22, RU620137 Ekaterinburg, Russia
²Ural Federal University named after the first President of Russia B.N. Yeltsin, Institute of Natural Sciences and Mathematics, Mira street 19, RU620026 Ekaterinburg, Russia
³Botanical Garden UB RAS, 8 Marta street 202a, RU620144 Ekaterinburg, Russia
*Correspondence: lili.khamidullina@gmail.com

Abstract:

Agricultural cultivation of astragalus is fraught with a number of difficulties caused by both certain requirements for climatic conditions and individual characteristics of plants of this genus. In this study, carboxyalkylated derivative of chitosan was first proposed to use for improvement of astragalus propagation. Effects of N-(2-carboxyethyl)chitosan on in vitro A. glycyphyllos and A. membranaceus seed germination and seedling growth and development in comparing with β-alanine and chitosan acetate were detected. Carboxyethylation of chitosan leads to an increase in hydrophilic properties of the molecule, which enhances a penetration of nutrients inside the plant owing to improved solvating effect and bioadhesive activity. Seed germination assay were performed on Murashige-Skoog growth medium with or without tested compounds. N-2-Carboxyethylated derivative of chitosan was found to demonstrate active stimulating effect on the plant growth and development, contrary to the effect of acetate chitosan, but not to cause an activating effect on seed germination, while β-alanine does.

Key words:

agricultural benefits, bioactivity, functional derivatives, morphological measurements, plant grow regulators, polysaccharides, statistical Friedman test, stimulating effect

The effect of the 1,2,3-triazolo[5,1-b][1,3,4]thiadiazines on Solanum lycopersicum L. seed germination

O.A. Vysokova¹, T.A. Kalinina¹, T.V. Glukhareva¹²*, A.A. Kochubei³ and O.A. Cherepanova³

¹Ural Federal University, Mira street 19, RU620002 Ekaterinburg, Russia
²Postovsky Institute of Organic Synthesis, Ural Branch of the Russian Academy of Sciences, Sofia Kovalevskaya street 22, RU 620990, Ekaterinburg, Russia
³Botanical Garden UB RAS, 8 Marta street 202a, RU620144 Ekaterinburg, Russia
*Correspondence: taniagluhareva@yandex.ru

Abstract:

In recent years chemical pesticides are being replaced by environmentally friendly and universal means of plant protection that are able to exert a complex effect on the plant (stimulate growth and development, improve metabolic processes, develop resistance to pathogens, etc.). The effect of new synthetic growth regulators of the 1,2,3-triazolo[5,1-b][1,3,4]thiadiazine class and trade phytohormones (6-BAP, GA and TDZ) on the growth processes, growth energy and vitality of tomato seeds, as well as morphological parameters of seedlings was researched in this article. It was revealed that the effect of synthesized compounds on seed vigor and viability of seedlings are superior to commercial phytohormones. In the early stages of germination the seed vigor of tomato seeds treated with the compounds TT1-TT5 were superior to one in treatments with phytohormones and in control experiment. As a result of the experiment, the most viable seedlings were formed into the treatments TT1, TT2 and TT3 (in all studied concentrations). Tomato seedlings treated with 6-BAP and GA at a concentration of 5 mg L^-1 produced the worst results.

Key words: