Enzymatic activity of podzolized chernozem contaminated by pollutants during its detoxification

O. Chernikova¹,*, Yu. Mazhayskiy¹ and L. Ampleeva²

¹Academy of law management of the federal penal service of Russia, Sennaya street 1, RU390036 Ryazan, Russia
²Ryazan State Agrotechnological University named after P.A. Kostychev, Kostychev
street 1, RU390044 Ryazan, Russia

Abstract:

The soil is an indicator of the general technogenic situation. In terms of the scale of pollution, as well as the impact on biological objects, heavy metals occupy a special place among pollutants. One of the priority pollutants are Pb, Cd, Zn, Cu. In assessing the ecological state of the environment, the study of the soil cover plays an important role. The most informative integral characteristics of the biological activity of the soil is the activity of soil enzymes. In a lysimetric experiment with podzolized chernozem, we studied the change in the biological activity of soil in terms of dehydrogenase, catalase, urease, invertase and phosphatase enzymatic activity under the complex influence of heavy metals under conditions of the use of detoxification agents. The soil at the experimental site had the following characteristics: pHKCl 6.2; humus content – 3.2%, P₂O₅-229 mg kg-1, K₂O-250 mg kg^-1of soil. Organic and mineral fertilizers in various combinations were used as detoxifiers. According to the obtained data, the redox enzyme – dehydrogenase and hydrolytic enzymes urease and phosphatase are the most sensitive to soil pollution. The best decontamination effect is obtained when using a system of organo-mineral fertilizers, what contributes to an increase in the activity of soil urease by 3.38 times, invertase – by 2.47 times, phosphatase – by 1.48 times, dehydrogenase – by 1.46 times, catalase – by 1.60 times. Changes in the activity of these enzymes can be used to diagnose the effectiveness of the use of various fertilizer systems on soil contaminated by heavy metals.

Key words:

catalase, dehydrogenase, detoxification, heavy metals, invertase, phosphatase, podzolized chernozem, urease

Humus content in a podzolized chernozem after a long-term application of fertilizers in a field crop rotation

H. Hospodarenko¹, I. Prokopchuk¹*, S. Prokopchuk¹ and А. Trus²

¹Uman National University of Horticulture, Faculty of Agronomy, Department of Agricultural Chemistry and Soil Science, 1 Instytutska street, UA20305 Uman, Ukraine
²Uman National University of Horticulture, Faculty of Engineering and Technology, Department of Applied Engineering and Labor Protection, 1 Instytutska street, UA20305 Uman, Ukraine
*Correspondence: pivotbi@ukr.net

Abstract:

The article presents the results of the research into influence of a long-term (50 years) application of different fertilizer rates and fertilizer systems in the field crop rotation on the humus composition and optical parameters of humic acids of a heavy- loamy podzolized chernozem of the Right Bank Forest-Steppe of Ukraine.
It was found that application of fertilizers significantly affects the dominance of humic acids over fulvic acids in the composition of soil, which indicates humate type of soil. Chroma index of humic acids is within 3.56–3.75 depending on a fertilizer. Indicators of the optical properties of humic acids of a podzolized chernozem have a high degree of humification.

Key words: