Laboratory seed germination of Hydrangea L. species depending on germination methods and genotype

M. Osipov, V. Polishchuk, А. Brovdi* and Y. Velychko

Uman National University, Faculty of Forestry and Landscape Gardening, Instytutska Str., UA20305 Uman, Ukraine
*Correspondence: abrovdi@ukr.net

Abstract:

The aim of the study was to determine the effect of germination methods and cultivar characteristics of plants of the genus Hydrangea L. on laboratory seed germination and to identify approaches for optimising germination protocols to obtain high-quality seeds. It was found that, on average across Hydrangea species, the highest seed germination (80%) was achieved on moistened filter paper, whereas the lowest germination (52%) was recorded using a paper substrate (two layers below and one above the seeds). Among all species, Hydrangea paniculata exhibited the lowest germination, ranging from 46% to 67% across the germination methods evaluated. The highest germination (87%) was recorded for Hydrangea macrophylla on moistened filter paper. Analysis of variance (ANOVA) showed that the factor ‘hydrangea species’ had the greatest effect on seed germination, accounting for 98.08% of the total variation. In both cultivars of Hydrangea paniculata, seed germination was significantly lower throughout the study than in the cultivars of Hydrangea macrophylla and Hydrangea arborescens, ranging from 64% to 68%. The results further showed that hydrangea seeds responded only slightly to pre-germination cooling. On average across species, the mean germination of non-cooled seeds was 80%. Cooling for 5 days reduced germination, whereas cooling for 10 and 20 days increased germination by only 1% relative to the non-cooled control. Irrespective of the treatment applied, the highest and nearly identical germination was observed in Hydrangea macrophylla and Hydrangea arborescens, whereas Hydrangea paniculata showed significantly lower germination.

Key words:

Agroecological substantiation of Medicago sativa cultivation technology

N. Telekalo* and M. Melnyk

Vinnytsia National Agrarian University, Faculty of Agronomy and Forestry, Soniachna street 3, UA21008 Vinnytsia, Ukraine
*Correspondence: nataliiatelekalo@gmail.com

Abstract:

Medicago sativa is one of the most productive legumes, which provides high protein production. Application of the biostimulant and microfertilizers is quite relevant and effective. Theoretically substantiates and presents ways to solve the scientific problem of formation of Medicago sativa productivity and the impact on them of seed treatment and foliar nutrition, taking into account the conditions of the Forest-Steppe right bank of Ukraine. The research was conducted in the research field of Vinnytsia National Agrarian University in the village of Agronomichne, Vinnytsia district during 2016–2018. Sinyukha variety was sown (2010). There has been established that the use of pre-sowing treatment of seeds and crops of Medicago sativa with biostimulant and microfertilizer improves the conditions of growth and development of the crop and increases individual productivity. On the researches it is established that fodder productivity of Medicago sativa sowing is provided by application of biostimulators of growth and microfertilizers on crops, genetic potential the culture realized as much as possible on the 2nd year of cultivation. The effect of growth stimulants on Medicago sativa productivity was defined and the optimal combination of macro- and microelements in modern complex fertilizers was determined, which contributes to the maximum possible yield of Medicago sativa green mass. New technological regulations for the use of bioorganic preparations and components in the technological scheme of Medicago sativa cultivation have been developed. It was found that on average for three years of vegetation of Medicago sativa sown on gray forest soils in the Forest-Steppe Right Bank, the highest yield of seeds and green mass provides the option of treatment with biostimulator Saprogum® and microfertilizer Wuxal®. Creating optimal conditions for mineral nutrition for Medicago sativa plants through the use of bacterial fertilizers and foliar nutrition contributed not only to the formation of high grain yields, but also significantly improved biochemical parameters and, consequently, increased crude protein content in grain, which is important for solving the problem of vegetable protein and balancing the diets of farm animals. The research results are aimed at solving current problems of technological renewal and development of agro-industrial complex on the basis of development of bioorganic models of varietal technology of Medicago sativa cultivation with orientation at the level of adequate arable productivity and climate fertility.

Key words:

cultivation, Medicago sativa, seed quality, technology, yield