The selection of maize parent lines within marker assisted selection (MAS) by crtRB1-3’TE marker for Steppe zone of Ukraine
¹Ukrainian Institute for Plant Variety Examination, Henerala Rodimtseva Str. 15, UA03041 Kyiv, Ukraine
²Research Institute of Agrarian Business, Tokova Str. 2A, UA52502 Vesele village Dnipro region, Ukraine
³National University of Life and Environmental Sciences of Ukraine, Faculty of Plant Protection, Biotechnology and Ecology, Heroiv Oborony Str. 15, UA03041 Kyiv, Ukraine
has a large genotypic diversity and a broad scale of economically important
traits. Therefore, it is extremely important for breeding to obtain hybrids which
can ensure high yield even under severe growing conditions, such as in Steppe
zone of Ukraine. This study aimed to determine the optimal allele ratio by
crtRB1-3’TE marker in parental components of maize modified hybrids. There were investigated four hundred sixteen maize
hybrids which are modified hybrids of heterotic model
(Iodent × Iodent) × Lancaster germplasm. SCA (specific
combining ability) effects for grain yield and grain moisture content were
calculated in maize hybrids with different allele combinations of crtRB1 gene.
As results, the stable positive SCA effects were calculated for
hybrids with allele combination
(296 bp + 875 bp) × 543 bp × 296+875 bp
during both 2019 and 2020 (1.23 and 0.99 t ha-1, respectively).
The lowest SCA effects for grain moisture content were obtained for modified hybrids with
allele combination (543 bp × 543) × 296 + 875 bp both in 2019 and 2020 (-0.54 and -0.36%, respectively). The greatest
influence SCA effects for grain yield had the interaction of allele
combinations and year weather conditions (39%),
the impact the allele combinations was 36%. The year weather conditions had the greatest impact on SCA effects for grain moisture content (44%), the allele combination (36%). Thus,
it was determined that SCA effects for studied indicators of heterotic model (Iodent × Iodent) × Lancaster under contrasting weather condition are resulted from both genotypes of hybrid parents and favourable allele presence.