Tag Archives: scanning electron microscopy

xxx S. Motyleva, E. Vlasova, N. Kozak, M. Gins and V. Gins
Morphological and anatomical characterization of Actinidia kolomikta (Rupr. & Maxim.) Maxim. (C3) and Amaranthus tricolor L. (C4) leaves
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Morphological and anatomical characterization of Actinidia kolomikta (Rupr. & Maxim.) Maxim. (C3) and Amaranthus tricolor L. (C4) leaves

S. Motyleva¹*, E. Vlasova¹, N. Kozak¹, M. Gins² and V. Gins²

¹Federal Horticultural Research Center for Breeding, Agrotechnology and Nursery, Zagorevskaj Str. 4, 115598 Moscow, Russia
²Federal Scientific Center of Vegetable Growing, Selectnaya Str., Moscow region, Odintsovsky urban district, 143080 VNIISSOK village, Russia
*Correspondence: motyleva_svetlana@mail.ru

Abstract:

Morphological and anatomical features of new cultivars with photosynthesis of C3 (Actinidia kolomikta (Rupr. & Maxim.) Maxim. cv. ‘Narodnaya’) and C4 (Amaranthus tricolor L. cv. ‘Valentina’) were established by light and scanning electron microscopy, as well as energy-dispersive analysis. The leaf lamina of Actinidia kolomikta cv. ‘Narodnaya’ has a dorsoventral anatomical structure, anomocytic stomata on the abaxial epidermis and two types of trichomes: multicellular, uniseriate hairs and multicellular bristle-like protrusions, containing raphids. The needle-like raphides are located in subepidermal layers along the veins. A vascular system of petiole consists of two upper concentric bundles and the crescentic vascular strand. A starch sheat is present. Raphides (needle-shaped and rectangular) are located in phloem and cortical parenchyma cells, contain Ca, K, Mg, P and Si. The leaf lamina of Amaranthus tricolor cv. ‘Valentina’ have the kranz-anatomy, dorsiventral mesophyll and contain druses. Betacyanins are concentrated in the epidermis and mesophyll, but are not present in the bundle sheath. The number of vascular bundles in petioles is odd-numbered and variable (from 5 to 13). Trichomes are multicellular, uniseriate, ending in a large oval cell. Cells with betacyanins are present in the epidermis cortex, and, rarely, the collenchyma and phloem of the petiole. Cells with betaxanthins are absent. A starch sheat is brightly pigmented with betacyanins. The crystall sand is deposited in the parenchyma cells of the cortex and pith of the petiole and contains Ca (mainly) and K oxalates. Druses in the leaf lamina additionally contain Mg and P.

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1014–1023 M. Hromasová, P. Kic, M. Müller and M. Linda
Evaluation of quality and efficiency of ventilation equipment by scanning electron microscopy
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Evaluation of quality and efficiency of ventilation equipment by scanning electron microscopy

M. Hromasová¹*, P. Kic², M. Müller³ and M. Linda¹

¹Czech University of Life Sciences in Prague, Faculty of Engineering, Department of Electrical Engineering and Automation, Kamýcká 129, CZ165 21 Prague, Czech Republic
²Czech University of Life Sciences in Prague, Faculty of Engineering, Department of Technological Equipment of Buildings, Kamýcká 129, CZ165 21 Prague, Czech Republic
³Czech University of Life Sciences in Prague, Faculty of Engineering, Department of Material Science and Manufacturing Technology, Kamýcká 129, CZ165 21 Prague, Czech Republic
*Correspondence: hromasova@tf.czu.cz

Abstract:

The aim of this research is an evaluation of the quality and function of ventilation equipment in basement rooms. There was analysed the function of ventilation system in relation to the quality of outdoor and indoor environment. The concentration of air dust was measured by exact instrument DustTRAK II Model 8530 aerosol monitor inside and outside the building. Using the special impactors the PM1, PM2.5, PM4, PM10 size fractions were also measured. Particles separated from the ventilation equipment were examined with SEM (scanning electron microscopy) using a microscope TESCAN MIRA 3 GMX. Obtained results of measurements were evaluated by statistical instruments and concentrations of different size of dust particles were analysed. The size of particles outlet the ventilation equipment was ca. of 55% lower than the size of the particles inlet the ventilation equipment. The difference in tested sizes of the dust particles in the ventilation equipment and outlet the ventilation equipment, i.e. in the place of cleaned air inlet into the basement room, was statistically proved. The diversity of impurities caught by the ventilation equipment and impurities moving in the air in the tested room is obvious from the results of SEM analysis.

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