The use of DNA markers for the evaluation of maize lines and hybrids based on cytoplasmic male sterility
¹Ukrainian Institute for Plant Variety Examination, Henerala Rodymtseva Str. 15, UA03041 Kyiv, Ukraine
²Research Institute of Agrarian Business, Berezynska Str. 80, UA49130 Dnipro, Ukraine
*Correspondence: prysiazhniuk_l@ukr.net
Abstract:
The use of cytoplasmic male sterility (CMS) is very important for the production of maize hybrids. The new inbred lines and hybrids of maize of Ukrainian breeding were studied. In the field, four pairs of sterile analogue of line RL106S were obtained during four backcrossing (17М, 19М, 23М, 27М and 29М) and maintainer lines of RL106fS (18М, 20М, 24М, 28М and 30М) for S type cytoplasm and RL108C (195С, 201С, 205С, 207С, 209С) і RL108fC (196С, 202С, 206С, 208С, 210С) for C type respectively. For S type, the following combinations were obtained: RL23S×RL106fS, RL107S×RL106fS, RL98S×RL106fS, RL105S×RL106fS, RL113S×RL106fS and for C type: RL109C×RL108fC, RL110C×RL108fC, RL112C×RL108fC, RL114C×RL108fC, RL115C×RL108fC. The obtained hybrid combinations were planted the following year in a control nursery for field trials. According to the results of the field assessment, all the hybrids were sterile. The types of sterility of the studied lines and hybrids were determined using polymerase chain reaction (PCR) with specific primers for C and S types of cytoplasms. The presence of specific amplicons 398 and 799 bp was determined in sterile lines with C and S types of cytoplasm, respectively. Amplicons 398 and 799 bp were identified in simple-cross and simple reconstituted hybrids on a sterile basis, and can be used to determine the type of hybrid and its maternal component at the stages of selection and examination of new hybrids.
Key words:
cytoplasmic sterility, DNA markers, Zea mays L.