Biochemical responses of 5 buckwheat (Fagopirum esculentum Moench.) cultivars to seed treatment by Azospirillum brasilense
¹National University of Life and Environmental Science, Department of Plant Science, Heroiv Oborony str., no 15, UA03041 Kyiv, Ukraine
²Institute of Cell Biology and Genetic Engineering of NAS Ukraine, Academika Zabolotnoho str., no 148, UA03043 Kyiv, Ukraine
*Correspondence: honchar@nubip.edu.ua
Abstract:
Cultivation condition have a large effect on efficiency of buckwheat. Drought, high temperatures and their fluctuations, salinity, oxygen deficit in the soil, ultraviolet radiation, and abnormal nutrient content in the soil are the most common reasons of decreasing productivity of plants. Suboptimal parameters of the cultivation technology can also cause abiotic stress. Plant can decrease its efficiency from 30% to 50% depend on stress conditions. Using bacterial cultures is one of the preventive approaches to overcoming the negative impact of stressors. Microorganisms produce biologically active substances that stimulate plant growth, increase their resistance to abiotic factors. They are growth regulators and long-acting anti-stressants as well. Malondialdehyde (MDA) is formed as a result of the oxidative degradation of polyunsaturated fatty acids. Fructans are polysaccharides that are derived from D-fructose residues found in higher plants, green algae and bacteria. Fructans are involved in the adaptation of plants to the action of abiotic stressors and are valuable nutrients. The effect of Azospirillum brasilense pre-sowing treatment of buckwheat seeds on physiological and biochemical processes of MDA and fructan content was researched. Seed treatment with Azospirillun brasilense reduced the content of MDA in Ukrayinka and Syn 3/02. Sofia and Olga had a low level of MDA, but seed treatment increase it. Seed treatment increased the efficiency of photosynthesis (Fv / Fm) in Syn 3/02 from 0.58 to 0.72; in other varieties this effect was negligible. All cultivars have a strong relation between MDA and fructan content, that shows their participation in responses on cultivation conditions. Efficiency of photosynthesis in flowering–seed formation stage (BBCH 65–75) was close to maximum in field condition(0.70 ± 0.05) and seed treatment can increase it.
Key words:
buckwheat, fluorescence, fructans, malondialdehyde (MDA)