Tag Archives: solid-state fermentation

1537–1546 R. Uvarov, E. Shalavina, A. Briukhanov and E. Vasilev
Aerobic solid-state fermentation of the solid fraction of pig slurry
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Aerobic solid-state fermentation of the solid fraction of pig slurry

R. Uvarov*, E. Shalavina, A. Briukhanov and E. Vasilev

Federal State Budgetary Scientific Institution “Federal Scientific Agroengineering Center VIM”, 3, Filtrovskoje Shosse p.o. Tiarlevo, RU196625 Saint Petersburg, Russia
*Correspondence: puo-24@mail.ru

Abstract:

Current expansion in the pig number in Russia and their concentration in separate locations contribute to higher environmental risks. One key risk factor is the slurry produced. To utilize it more efficiently, many pig farms introduce its solid-liquid separation. The study objective was to explore the feasibility of accelerated aerobic solid-state fermentation of the solid fraction of pig slurry in closed installations. The stable thermophilic process under the temperature above +55 °C achieves shorter processing time of 3–4 days. To date, however, there is no representative evidence of such an experience. Four options of fermented mixture composition were tested based on two types of the solid fraction of pig slurry: Type 1 fraction coming from a screw separator and Type 2 fraction coming from a decanter centrifuge. The fermenter operating modes were tested in the authors’ previous studies associated with processing of the solid fraction of cattle manure and bedding poultry manure. The intensity measure of fermentation was the temperature reached by the processed material in the fermenter. Under the investigated operation modes, the stable temperature was observed for nine days in the mesophilic process: 20 °С to 55 °С; in some cases, the transition to the thermophilic process – above 55 °С was recorded. Adding the catalytic components to the processed material accelerated the substrate self-heating and a higher temperature up to 59 °С was reached. This suggests that the considered operating modes of the fermenter were suitable for the fermentation of the specified substrate.

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290–300 E. Serba, N. Pimenov, P. Mochalina, M. Overchenko, Yu. Borscheva, A. Sharikov and L. Rimareva
Production of Aspergillus oryzae RCAM 01133 biomass with increased protein and polysaccharides content using by-products of food industry
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Production of Aspergillus oryzae RCAM 01133 biomass with increased protein and polysaccharides content using by-products of food industry

E. Serba¹, N. Pimenov², P. Mochalina¹, M. Overchenko¹, Yu. Borscheva¹, A. Sharikov¹* and L. Rimareva¹

¹Russian Research Institute of Food Biotechnology – branch of Federal Research Center of Nutrition and Biotechnology, Samokatnaya street 4b, RU111033 Moscow, Russia
²Federal State Educational Institution of Higher Professional Education Moscow State Academy of Veterinary Medicine and Biotechnology named after K.I. Skryabin, 23 Academician Skryabin street, RU109472 Moscow, Russia
*Correspondence: anton.sharikov@gmail.com

Abstract:

The biomass of filamentous fungi is a promising source of protein and carbohydrate. It can be used as an unconventional substrate in technologies for obtaining food and feed ingredients. The studies show that the fungus Aspergillus oryzae synthesizes an insufficient level of protein in the process of solid-state fermentation. The aim of the research was to develop conditions for the production of fungal biomass with a high content of protein and polysaccharides on the basis of solid-state fermentation using by-products of food industry as inexpensive substrate for biomass production. Wheat bran, soybean meal, distilled dry grains with solubles, and brewer’s spent grain were used as raw material. Results of fermentation show that the protein content was 73.4% and 82.0%, which is more than 3 times higher than in fungus grown by submerged fermentation. The studies of the biosynthesis properties of the selected strain A. oryzae RCAM 01133 confirmed the high biological value of microbial biomass cultivated using food by-products. Fermentation of micromycete on culture media containing soybean meal and DDGS provided an increase of protein content by 1.45 times. Maximum increase of amino acids was observed for isoleucine, leucine, tryptophan, and glutamic acid. The increased content of synthesized polysaccharides related to media containing wheat bran and DDGS. The highest concentrations of polysaccharides were 27.9% and 32.9%, respectively

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