The use of SSR-markers in rice breeding for resistance to blast and submergence tolerance

E. Dubina¹²*, P. Kostylev³, S. Garkusha¹², M. Ruban¹, S. Lesnyak¹, Y. Makukha¹, S. Korzh¹, D. Nartymov¹ and O. Gorun¹

¹Federal Scientific Rice Centre, Laboratory of Informational, Digital and Biotechnologies, Belozerny 3, RU350921 Krasnodar, Russia
²Kuban State Agrarian University, Faculty of Agronomy, Department of Biotechnology, Biochemistry and Biophysics, Kalinina 13, RU350004 Krasnodar, Russia
³Agricultural research center ‘Donskoy’, Laboratory of Rice Breeding and Seed Production, Nauchny Gorodok 3, RU347740 Zernograd, Russia
*Correspondence: lenakrug1@rambler.ru

Abstract:

The identification of effective specialized DNA markers providing the clear control of target locus inheritance by the trait of submergence tolerance has been conducted. Among the studied set of microsatellite markers, two the most informative SSR-markers – RM 7481, PrC3 showed high efficiency in detecting intraspecific polymorphism of rice varieties and lines used in the work. With the use of these markers the clear genotype marking the obtained hybrid rice plants by this trait has been conducted and it is has been verified by phenotype evaluation as a result of laboratory trials. The plant samples carrying the target gene in heterozygous and homozygous state has been selected. About 400 backcrossed self-pollinated rice lines with introgressed and pyramided resistance genes Pi-1, Pi-2, Pi-33, Pi-ta, Pi-b to Pyricularia oryzae Cav. were obtained within the frameworks of program to develop genetic rice sources resistant to blast. The conducted testing for resistance to blast and the assessment by economically valuable traits have allowed to select the prospective rice samples. The plant samples of F2 and BC1F1 generations with combination of resistance to blast genes (Pi) and submergence tolerance gene (Sub1A) in homozygous and heterozygous state that is confirmed be the results of analysis of their DNA have been obtained. The obtained hybrid plants are being tested in breeding nurseries for a complex of economically valuable traits. The best plants will be selected and send to State Variety Testing system. Their involving in rice industry will reduce the use of plant protection chemicals against diseases and weeds, thereby increasing the ecology status of the rice industry.

Key words:

blast resistance genes, DNA markers, PCR analysis, rice, rice varieties, submergence tolerance genes

The use of DNA markers for the evaluation of maize lines and hybrids based on cytoplasmic male sterility

L. Prysiazhniuk¹*, Y. Honcharov², S. Chernii¹, S. Hryniv¹ and S. Melnyk¹

¹Ukrainian Institute for Plant Variety Examination, Henerala Rodymtseva Str. 15, UA03041 Kyiv, Ukraine
²Research Institute of Agrarian Business, Berezynska Str. 80, UA49130 Dnipro, Ukraine
*Correspondence: prysiazhniuk_l@ukr.net

Abstract:

The use of cytoplasmic male sterility (CMS) is very important for the production of maize hybrids. The new inbred lines and hybrids of maize of Ukrainian breeding were studied. In the field, four pairs of sterile analogue of line RL106S were obtained during four backcrossing (17М, 19М, 23М, 27М and 29М) and maintainer lines of RL106fS (18М, 20М, 24М, 28М and 30М) for S type cytoplasm and RL108C (195С, 201С, 205С, 207С, 209С) і RL108fC (196С, 202С, 206С, 208С, 210С) for C type respectively. For S type, the following combinations were obtained: RL23S×RL106fS, RL107S×RL106fS, RL98S×RL106fS, RL105S×RL106fS, RL113S×RL106fS and for C type: RL109C×RL108fC, RL110C×RL108fC, RL112C×RL108fC, RL114C×RL108fC, RL115C×RL108fC. The obtained hybrid combinations were planted the following year in a control nursery for field trials. According to the results of the field assessment, all the hybrids were sterile. The types of sterility of the studied lines and hybrids were determined using polymerase chain reaction (PCR) with specific primers for C and S types of cytoplasms. The presence of specific amplicons 398 and 799 bp was determined in sterile lines with C and S types of cytoplasm, respectively. Amplicons 398 and 799 bp were identified in simple-cross and simple reconstituted hybrids on a sterile basis, and can be used to determine the type of hybrid and its maternal component at the stages of selection and examination of new hybrids.

Key words: